Inhibition Of CD24 Expression To Induce Anoikis In Ovarian Cancer Cells

Objective: To explore the effects of CD24 on anoikis in ovarian cancer cells. Methods: The expressions of CD24 in ovarian cancer cell lines with highly and lowlymetastases were examined by RT-PCR. Cells were cultured with or without CD24 antibody in suspension to observed aggregates-formation. The effects of CD24 on anoikis were detected by colony-formation of cells in soft agar and flow cytometry in suspension culture.Results: The CD24 mRNA levels were significantly greater in highly metastatic cell line HO-8910PM (IOD 23210±347) compared with A2780 (IOD 6089±184) (P<0.01). The HO-8910PM formed larger and more aggregates in polyHEMA suspension and in soft agar than A2780 (HO-8910PM:35±1.2;A2780:16±1.5).And the latter was more sensitive to anoikis.(HO-8910PM 5.6±0.61%;A2780(16.0±0.65% ). (P<0.01).After blocked CD24 with antibody, the ability of these two cells to form aggregates were declined(HO-8910PM:19±3.0;A2780:8±2.0) and the anoikis increased (HO-8910PM 22.4±1.05;A2780 25.0±2.29)(P<0.01).Conclusions: The overexpression of CD24 in highly metastatic cell line of ovarian cancer may play important role in anoikis-resistance.