Ras Protein Contributes To Cerebral Vasospasm In Rabbits SAH Model

Objective Make the rabbit cerebral vasospasm model of subarachnoid hemorrhage. To study changes in rabbit models of behavior scores, the degree of cerebral vasospasm, expression of Ras-GTP and ERK1/2 phosphorylation, apoptosis of endothelial cells.Methods The rabbit cerebral vasospasm model of subarachnoid hemorrhage, with the method that injection blood into the cisterna magna twice. Experimental groups: the first large group was divided into control group, SAH group, SAH + DMSO group, SAH + FTI-277 group; the second largest group was divided into control group, SAH 3 days group, SAH 5 days group, SAH 7 days group.①Evaluation of behavioral changes in rabbit model;②Measure cross-sectional area of vascular changes by computer software;③Detect Phosphorylation of ERK1/2 levels by immunohistochemistry;④Detect expression Ras-GTP and phosphorylated ERK1/2 by WESTERN BLOT;⑤Detect apoptosisr of abbit cerebral vascular endothelial cell by TUNEL.Results①SAH group, SAH+DMSO group than SAH+FTI-277 group behavior score lower, statistically significant difference.②SAH group, SAH+DMSO group and SAH+FTI-277 group was statistically significant cerebral vascular cross-sectional area, SAH+FTI-277 group and control group was not statistically significant.③SAH group and SAH+DMSO group deeper immunohistochemistry (phosphorylation of ERK1 / 2 levels higher), SAH + FTI-277 group and control group phosphorylation of ERK1 / 2 levels lower.④the second large group, SAH group and SAH+DMSO group, Ras-GTP and phosphorylated ERK1/2 levels increase significantly, are different from the control group and SAH + FTI-277 group; the second largest group, SAH 3 days group and SAH 5 days group's Ras-GTP and phosphorylated ERK1 / 2 levels increase, SAH 7 days group's Ras-GTP and phosphorylated ERK1 / 2 levels decreased.⑤SAH group and SAH + DMSO group more cerebral vascular endothelial cell apoptosis. Control group and SAH + FTI-277 rare apoptotic cells.Conclusion Production of cerebral vasospasm after subarachnoid hemorrhage rabbit model is satisfactory in the experiment. After SAH, Ras-GTP and phosphorylation ERK1/2 both increase significantly. The activation levels in time with the pathophysiology of cerebral vasospasm corresponding changes. This suggests that Ras in the pathologic process plays an important role.