Department Of Respiratory Medicine, Union Hospital Of Tongji Medicine College, Huazhong University Of Science And Technology

Objective To investigate the expression of Kv1.3 and Kv1.5 channels in rat macrophages cell line NR8383 cells under the stimulation of bleomycin.Methods: Cultured the NR8383 cells in vitro. Then,(1) five grouped cells were treated with BLM in five different drug concentrations ( 0,0.1,1.0,10,100 mU/ml ); (2) treated cells in four groups with NS, DEX, DEX+BLM and BLM, respectively. Following above, detected the mRNA and protein of Kv1.3 and Kv1.5 channels by Real time PCR and Western blot.Results: (1) Kv1.3 and Kv1.5 expressed in the NR83683 cells. (2) Compared with the control group, the expression of Kv1.5 channels increased under the stimulation of bleomycin.Conclusion: Kv channels in alveolar macrophages may play a role in lung fibrosis induced by bleomycin.