Study On Extraction And Separation Technology Of Effective Collstituents And Comprehensive Utilization From The Dregs Of The Angelica Sinensis

In this article, the extraction and purification craft of polysaccharide and ferulic acid from the dregs of Angelica sinensis(Oliv.)Diels were researched and optimized, and the pharmacological action of polysaccharide was researched . The objective of research is to provide the technical program and empirical datum for redevelopment use of Angelica sinensis.1. Through exploring the polysaccharide extraction and purification craft from the dregs of Angelica sinensis, it was determined the best route of water-extraction and alcohol-precipitation by the single factor experiment and the orthogonal test. Optimum parameters for the extraction with water is as follow: temperature100℃,time2h , solid-liquid ratio 1:8 and frequency ratio 3. then the extracting solution was condensed to the cocnentration factor 1:1and injected 95% alcohol to make the alcohol concentration to 80%. Thick polysaccharide was settled by offcenter under the low temperature for 6h.2. In the polysaccharide purification process, the protein-free number of times and the decolorization was researched. It was determined 3 times for protein-free number by the Sevag method and 0.1 % activated carbon for decolorization. It was obtained two kinds of purification polysaccharides from the thick polysaccharide by fractional precipitation, and determined the contents and rates of the three kinds of polysaccharides by the Phenol-sulfuric acid method, the thick polysaccharide content is 50.84 % and rate is 10.73%; the content of angelica purification polysaccharideⅠis 62.84%, rate is 7.84%; the content of angelica purification polysaccharideⅡis 69.16%, rate is 3.33%. The protein content of three kinds of polysaccharides is respectively 19.49 %, 17.11 % and 15.86% by Bradford method.3. Uniform design was adopted to observe the effect of alcohol density, temperature, time and solid-liquid ratio on ferulic acid extraction rate, and optimum parameters for the extraction with alcohol is as follow: temperature85℃, time2.5h, solid-liquid ratio1:16 and frequency ratio 3. The content of ferulic acid is 0.04247 mg/g under this condition.4. Three kinds of polysaccharides from the dregs of Angelica sinensis can control ear edema in mice which was casued by dimethylbenzen and the blood vessel permeability by the acetic acid-induced, which is indicated that the angelica polysaccharides have certain antiinflammatory effect, especially the quantity effect relations of the angelica purification polysaccharideⅠis more remarkable.5. Angelica purification polysaccharideⅠwas carried on the immunization experiment, the test result indicated that it can increase ctx-induced Phagocytosis index, Thymus index and Spleen index, and can increase the mouse blood serum hemolysin value. so the angelica purification polysaccharideⅠhas certain effect in immunization.