Expression And Clinical Significance Of CADM1 & DAL-1/4.1B In Colorectal Carcinoma

Aims:To investigate the expression and clinicpathological significance of CADM1 and DAL-1/4.1B proteins in colorectal carcinoma,colorectal adenoma and normal colorectal mucosa specimens and its possible implications.Methods: Immumohistochemical staining was performed to detect the expression of CADM1 and DAL-1/4.1B proteins in 30 cases of colorectal carcinoma tissues, 10 cases of colorectal adenoma tissues, and 30 cases of normal colorectal mucosa tissues.Western-blot was used to measure the protein expression of CADM1 and DAL-1/4.1B in 30 cases of colorectal cancer tissues, 10 cases of colorectal adenoma tissues, and 30 cases of normal colorectal mucosa tissues.Semi-determination was performed by image analysis software.Results:The positive rate of CADM1 and DAL-1/4.1B showed downward trends in cancer tissues, colorectal adenoma tissues and normal control. Positive rate of CADM1 and DAL-1/4.1B was 23.33%/26.67% in cancer tissues, and 70%/70% in colorectal adenoma tissues, 86.67%/83.33% in normal control. The Western-blot band shows that the gray level of CADM1 and DAL-1/4.1B in cancer tissues is significantly lower than in colorectal adenoma tissues and normal control(0.229±0.053/0.223±0.052 Vs 0.299±0.068/0.291±0.066,0.229±0.053/0.223±0.052 Vs 0.418±0.081 /0.333±0.061, P<0.05). Expression and location of CADM1 protein were parallel and relevant with the expression of DAL-1/4.1B , the immunoreactive intensity of the two proteins was similar. Semiquantitative analysis showed that there was a positive correlation between the expression of CADM1 and DAL-1/4.1B(r=0.710, P<0.05). No relationship was found between expression of CADM1 and DAL-1/4.1B in age or histological differentiation and gender(P>0.05). But expression of CADM1 in Dukes stage C+D group was much lowher than Dukes stage A+B group (P<0.05).Conclusions: Downregulation of CADM1 and DAL-1/4.1B proteins expression are exist in colorectal carcinoma. The inactivation of CADM1 and DAL-1/4.1B cause the growth and development of colorectal carcinoma. CADM1 and DAL-1/4.1B are interact with eachother through this progress.