Lung Protection Of Bradykinin Beta 2 Receptor Antagonist During Post-lung Transplantation With Ischemia/Reperfusion Injury

Objective: To investigate the protective effects of HOE-140 on transplanted lung in rats single lung transplantation model[1].Methods: Pure SD rats were divided into three groups,donor and receptor were syngeneic rats(SD to SD).Orthotopic lung transplantation model was used.Grafts of control groups were flushed and preserved in LPD-Glucose exposed to 18 h of cold ischemia before transplantation. Peak airway pressure (PAP),blood gas analysis(PO2),wet/dry weight ratio(W/D),the activity of myeloperoxidase(MPO)and the contents of MDA,CON were analyzed at 1 day after transplantation. Experimental groups(EXP1 and EXP2) were divided into two groups, grafts were perfused and stored for 18h with LPD-Glucose plus DPPIV (AB192)catalytic inhibitor, the pulmonary function were detected as control groups at 1 days post-transplantation, respectively. EXP2 was given HOE-140 30 minutes before is chemia. Histopathological studies were also done in the same locus of lung specimen and HE staining for HOE-140 were analyzed[1]. Result:Experimental groups versus CON showed lowered PAP(P<0.01),better PO2 (P<0.05),less wet/dry weight ratio(P<0.05),decreased MPO enzymatic activity and lower accumulated MDA(P<0.05),the differences had statistical significance. Moreover, histological and immunological tissue edema was mild in the experiment group[1].Conclusion:The bradykininβ2 receptor antagonist HOE-140 can ameliorate IRI in lung grafts efficiently, and improve the lung function[1].