| Objective:The Mechanism of Study On Cervus and Cucumis Polypeptide Injection on Promoting Chondrocytes Proliferation with Knee Osteoarthritis。Methods:The Experimental study:The 50 New Zealand white rabbits were randomly divided into model group of 35. sham module 8, the normal group of 7。Hulth model modeling method group made reference to osteoarthritis animal models, fake modules for the left only after the skin incision medial suture, normal group without any surgical treatment。After 4 weeks in each group randomly selected from two rabbits were observed under light microscope and morphological changes of articular cartilage detected by ELISA in synovial fluid cytokine IL-1β,TNF-αlevels。Successful model after the model group were randomly divided into treatment group and control group and blank group。Treated group Injection 0.5ml Lugua polypeptide articular injection of evey day; The control group for every two-dimensional Glucosamine orally; 0.5ml normal saline blank group articular injection of 1 day。Were taken after injection one day,15 and 30 days and synovial fluid of three groups for the articular cartilage。Double antibody sandwich ELISA assay in synovial fluid cytokine IL-1β,TNF-αlevels and the level of TGF-β1。Observed by HE staining of histological changes of articular cartilage, transmission electron microscopy to observe the structural changes in articular cartilage cells; Detected by immunohistochemistry PCNA expression in articular chondrocytes was observed Lugua peptides promote cell proliferation of articular cartilage situation.。The Clinical observation:After the classification of 50 cases of early and mid knee osteoarthritis were randomly divided into treatment group,the control group on average。Line treatment group Lugua polypeptide 12ml daily in 0.9% sodium chloride injection in the intravenous infusion of 250ml of a course of treatment (15 days)。Glucosamine control group was treated every two-dimensional (tid), in all 30 days。Intervention were taken after 1day,15 and 30 days and synovial fluid of patients with two groups by double antibody sandwich ELISA assay in synovial fluid cytokine IL-1β,TNF-a levels and the level of TGF-β1o Peptides on the above observation Lugua regulation of cytokines, and then analyzes Lugua peptide injection on cartilage cell proliferation in patients with KOA situation。Results:The Experimental study:After 4 weeks of modeling, light microscope morphological changes of articular cartilage in model group, after the success of the modeling group and blank control group. Their IL-1βand TNF-a levels in different periods of 15 and 30 days higher than the treatment group o Between the two groups have a more significant difference (P<0.01); The TGF-β1 levels at different periods of 15 and 30 days were lower than the treatment group, two groups have a more significant difference (P<0.01); the treatment group, the cartilage cell proliferation with the control group and blank group, the proliferation of cartilage cells The comparison of the significant difference (P<0.01)o HE staining:30 days control group visibility in most of the cartilage cell lacunae disappeared, and the rough edge of serious damage, and cartilage cell proliferation significantly; 30 days in the control group some visibility within the cells of cartilage lacunae disappeared, there is destruction and rough edges of the situation, but also a small amount of cartilage cell proliferation; The treatment group in 30 days, clearly visible cartilage, cartilage cells arranged in neat rows, tidal line complete, there was no proliferation。TEM:The blank group in 30 days of its cytoplasmic shrinkage, chromatin was heterogeneous, cell apoptotic body formation; The control group in 30 days, there is a small amount of its uneven chromatin. some cells were apoptotic body formation; Treatment group at 30 days may be reflected in its cell surface microvilli more cartilage in membrane, the nucleus is more complete, more cytoplasm of rough endoplasmic reticulum in mitochondria, chromatin showed a more uniform distribution of the phenomenon。The Clinical observation:the treatment group of cytokines IL-1β,TNF-a levels in 15 and 30 days after drug intervention in different periods were lower than the control group, there were significant differences between the two groups (P<0.01); the TGF-βl level of 15 and 30 days in different periods were higher, there were significant differences between the two groups (P<0.01)。The clinical symptoms of the treatment group better than in the control group, there is a marked improvement in clinical symptoms。Conclusion:CCPI may be reduced by induction of cytokines in synovial fluid IL-1β,TNF-a levels and increased TGF-β1 level mechanisms to promote the role of knee osteoarthritis cartilage cell proliferation。... |
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