Analysis Of Genetic And Environmental Factors For H-type Hypertension In Anqing Rural Community

ObjectiveCardiovascular disease and cerebrovascular diseases have become a top dangerous "killer" to crisis human life worldwide,and indentified as main reasons for mortality and disability,Especially along with the rising living standard of the people, Because of some adverse events related with disease of cardiovascular and cerebrovascular are having a rising trend, thereinto stroke has become the first cause of death in rural areas, which seriously affect the quality of people's life Hypertension as a primary risk factor for cardiovascular disease and cerebrovascular disease has been accepted by the world. But in recent years, study of the relations of between Homocysteine (Hcy) and disease of cardiovascular and cerebrovascular which is relatively concentrated is become the focus of attention. Following the concept of essential hypertension with hyperhomocysteinemia has been defined as "H-type hypertension" which put forward by Dayi Hu et al in 2008, the research about it gradually goes deep. We proceed the ways of biochemical studies,determined genetic and asked a questionnaire to the primary hypertensive patients in Baihu,Tanggou and Oushan towns of Anqing city, Anhui Province, To explore the risk factors on H-type hypertension risk in the rural areas of Anqing. MethordForm 2007-7 to 2008-10 screened the primary hypertensive patients in Baihu,Tanggou and Oushan towns of Anqing city, Anhui Province, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to detect C677T polymorphism in N5,10-methylenetetrahydrofolate reductase (MTHFR) gene. Based on the known MTHFR genotypes, we then enrolled 241 cases of hypertension by three types of genotypes with even proportion of 1:1:1. Plasma homocystiene (Hey) level was determined by application of HPLC with fluorescence. Simultaneously blood pressure and body mass index (BMI) were also taken measurements, A questionnaire for the collection of age and cigarette and alcohol consumption status.Results1 There were CC, CT, TT three genetic polymorphism in MTHFR C677T genotype of the essential hypertension in Anqing city2 From the baseline data shows, the Body-mass index of men was higher than females(P<0.05),and the number of people in drinking and smoking in men was greater than in females(P<0.01).3 The average of Hey level in men was significantly greater than in females (9.7±4.7μmol/L VS12.8±7.2μmol/L,P<0.01) in Anqing rural community;4 The subjects with MTHFR TT genotype had a significantly higher average level of Hey than those with CC and CT genotypes (P<0.01), There were significant differences after adjusting the related risk factors also(P<0.01).5 Prevalence of H-type hypertension was up to 44.4%. percentage of H-type hypertension in men was signicantly greater than in females (60.3% VS 29.6%, P<0.05), among TT genotype in male rates as high as 80.6%.6 The risk of H-type hypertension in TT genotype was pronouncedly higher than in CT and CC genotypes(P<0.01), At the same time in every genotype internal male ratio markedly higher than women.7 There had interactions in the Hcy levels between gender and genotype, but but the effect was not significant before and after adjusting the related risk factors(P>0.05). 8 multiple linear/logistic regression models with adjustment for multivariates didn't identified the marked association of Hey level or H-type hypertension risk with other environmental variables including alcohol drinking, cigarette smoking, age, BMI, and baseline DBP and SBP(P>0.05).Conclusions1 The average of Hey level in primary hypertensive patients in Anqing city, Anhui Province is significantly greater than normal level and the Hey level in men is greater than in females, TT genotype is pronouncedly higher than in CT and CC genotypes;2 The risk of H-type hypertension in men higher than women and TT genotype is pronouncedly higher than in CT,CC and CC+CT genotypes, Meanwhile in genotype internal the male proportion is higher than women;3 There have interactions in the Hey levels between gender and genotype, but but the effect is not significant in this study;4 Don't identified the marked association of H-type hypertension risk with environmental variables including alcohol drinking, cigarette smoking, age and BMI.In conclusion, our present study suggested that the MTHFR C677T genetic variant and gender may be stronger predictors to Hey level and H-type hypertension risk, but not for drinking, smoking, age, body mass index and blood pressure, in Anqing rural community. In addition, there have interactions in the Hey levels between gender and genotype, but not significant.