Study On Expression And Significance Of IFITM1 Gene In The Tissue And Cell Of Cervical Cancer

Objective: To explore the transcription level and translation level of expression and clinical significance of IFITM1 gene in Uigur Cervical Squamous Cell Carcinomas tissues and normal Uigur Cervical tissues, to study of IFITM1gene on the growth of HeLa cells , apoptosis of HeLa cell by using gene transfection.Methods: IFITM1 protein expression level in Uigur cervical tissues was detected by immunohistochemistry(S-P Method).To detect IFITM1gene transcription level in 5 cervical squamous cell carcinoma and corresponding normal cervical tissues by real time PCR(RT-PCR).Construction the eukaryotic expression vector of IFITM1 gene,this recombinant was transfected into HeLa cells by using liposome transfection,to observe the number of cell growth by MTT Method and the cell apoptosis were examined by Annexinⅴ/PI double stainning flow cytometry.Results: (1)The Strong positive percentage of IFITM1 protein in Uigur cervical squamous cell carcinomas tissues was 15.0%(13/23)and 56.5% in chronic cervicitis immunohistochemistry method, it was significant difference (P<0.05). IFITM1 protein expression level in the hierarchical pathology of the cervical squamous cell carcinoma of Uigur women were not significant difference(Z=3.344,P>0.05) by non- parameter analysis. It indicates the expression of IFITM1 protein were not correlated with the hierarchical pathology of the cervical squamous cell carcinoma of Uigur women .(2) In 5 cervical squamous cell carcinoma tissues of the Uigur women, the maxlmum of IFITM1 gene expression at mRNA level is 134.95,the minimum is 22.34,the average expression level is 64.79±42.71. However, among 5 corresponding cervical normal tissues, the maximum of IFITM1 gene expression at mRNA level is 300.16,the minimum is 49.69 and the average expressed level is 128.29±88.77. There were significant difference(P<0.05) between Uigur Cervical Squamous Cell Carcinomas tissues and corresponding normal Uigur Cervical tissues by test of homogeneity of varianc and mean square interclass analysis (t=-2.497,p=0.019).(3) To construct the eukaryotic expression vector of IFITM1gene and transfected into HeLa cells. mRNA expression level were examined by RT-PCR method and protein expression level were detected by immunohistochemistry to ensure transfected successfully. The survial of cells after IFITM1 gene of pcDNA3.1 constructs transfected of HeLa cells were lower than pcDNA3.1 plasmid transfected in HeLa cells and non-transfected HeLa cells. With transfection experiment, the apoptosis rate of HeLa cells in non-transfected HeLa cells, pcDNA3.1 plasmid in HeLa cells and in IFITM1 gene of pcDNA3.1 recombinant plasmid in HeLa cells were 0.3307±0.0398, 0.4323±0.0224 and 0.4236±0.0287. Among IFITM1 gene of pcDNA3.1 constructs, pcDNA3.1 plasmid and non-transfected HeLa cells transfected into HeLa cells, the apoptosis rate were 27.3445±10.0532, 13.4664±3.6965 and 13.1607±2.4171, respectively. There were significant difference among three groups.Conclusion:IFITM1 gene expression at mRNA and protein level were decreased in cervical squamous cell carcinoma of Xinjiang Uigur women; IFITM1 gene may inhibit the growth of HeLa cell and inducing HeLa cell apoptosis.