The Extraction Of Curcumin And Biological Activity Of Its Derivatives

Curcumin is regarded as a natural compound which is extracted from plant. The variety of pharmacological effects of curcumin has been recognized, and it has been widely used in biomedical field. Curcumin not only can be the role of cancer prevention, but also can treat cancer. In addition, curcumin can treat atherosclerosis, liver fibrosis and acute lung injury and so on. Currently, curcumin is extracted by acid, organic solvent, enzymatic. The methods may be damage the structure of curcumin and the operation is cumbersome. The data processing is not conducive to analysis curcumin correctly. Further more, there are many defects in its structure, such as, the water solubility and bioavailability of it is low, and the half-life is short in vivo. All of them suggest that curcumin can not be used for biomedical applications easily.In this study, curcumin is extracted by using ethanol and water. After being filtered, curcumin is separated by column chromatography. Monosaccharides and vitamin compounds are used to modify curcumin. In order to esterified the phenolic hydroxyl of curcumin. The apoptosis of tumor cell is mediated by the receptor on tumor, In this process the compound which preparaed enhance the effect. The advantages of curcumin and its analogues are discussed about the toxicity in cells.(1) Curcumin is extracted by 5 different concentrations of ethanol from Curcuma longa L. Under the conditions of 80% ethanol, the yield of curcumin is up to 31.6% by HPLC. After using column chromatography, curcumin can be obtained,and the purity of it is high. The form of curcumin is orange crystalline powder.(2) Glucose, D-Arabinose and Nicotinic acid are used to modify curcumin. Three kinds of curcumin analogues are obtained. Both the electron withdrawing group of substituents and the ortho-effect of hydroxyl on benzene are benefit for esterification. Ester can be found on the sites of phenolic hydroxyl by using By IR, UV and magnetic resonance. The yield of target product is up to 30%. The absorption peak of C=O at 1850-1600cm-1 is higher compare with curcumin. With the increase of chromophore, blue shift is observed clearly. The characteristic peaks ofδ5.22(2H, d, J =7.6 Hz, Glca-1, Glcb-1),4.41(1H, d, J=5.8 Hz,2xAra-1) suggest that the target compounds have been prepared(3) When target compounds have been prepared, they will added to HeLa and HepG2 cell culture medium. We find that two kinds of tumor cells are inhibition in different concentrations of curcumin and its analogues.The products which are modified by Glucose and Nicotinic acid appear strong inhibition of tumor cells. According to the literature, they are associated with the cells apoptosis. Nicotine, a major component of tobacco, stimulates proliferation through nicotinic acetylcholine receptor (nAChR)-mediated signals. Activation of peroxisome proliferator-activated receptorγ(PPARγ) has been shown to inhibit tumor cell growth. Glucose receptor which is on the tumor cell surfaces can distinguish the coupling of glucose. In this way, the activity of curcumin will be enhanced, and the original groups are not destroyed. Further more, it is also more precisely to target the tumor cells.