Measurement Of HA Content In Influenze Vaccines Based On Image Processing

Using single radial immunodiffusion method to measure the hemagglutinin antigen (HA) content of influenza vaccines, the key is to determine the proliferation ring diameters. Automatic measuring system based on image processing for HA content is studied in this article. The system gets proliferation ring image by CCD camera, caculates the proliferation ring diameters by image processing such as sub-pixel edge detection, hough transform, and caculates the HA content by curve fitting. Thus, it realizes the operations' automation and intelligence for acquisition of proliferation ring diameters data, calculation of HA content and analysis of results, that effectively improves the measurement accuracy, and eliminating the human disturbance. This article describes the measuring principle of HA content and the design of automatic measuring system, focused on the study of proliferation ring detection algorithm. Finally, experiment results and system accuracy are showed and analysed.Experiments show that the system is suitable for every kinds of HA content, the sub-pixel detection accuracy of the proliferation ring can rearch 0.1 pixel, the concentration measurement accuracy is up to 2μg/ml, with relationship coefficient more than 0.99, and high stabilization of less 1% coefficient of variation, that meets the measurement needs.