| Human Cytomegalovirus(HCMV) is the most common virus of intrauterine infection。The virus often displays activity in infants,leads to multisystem and multiorgan's injury。The process how it results in chronic infection and affect multiorgans involves many links of immunological network。Exosome comes from the vesicles in the multivesicular bodies(MVBs) of many kinds of eukaryotic cells,whose function is determined by the source cells.They can mediate many immunologic mechanisms, and have close connection with virus infection。Moreover, some cytokines also paly important roles in the process of resisting virus infection。SO So we studied the quantity of exosome,IL-4 and IFN-γlevels in serum and/or exosome as well as dipeptidyl peptidase IV's activity of cytomegalovirus infection infants,to investigate the influence of CMV on infant's immune function,and the role of exosome and cytokines which play in the process of CMV infection.Materials and methods1.Study subjects1.1 Experimental group:Fifty-three cytomegalovirus infection infants were collected in the department of of QiLu hospital of Shandong university。The diagnostic criteria based on《The diagnostic project of cytomegalovirus infection》which was instituted by the digestion-infection group of pediatrics in Chinese Medical Association in Nov.1998, depleting other virus infection as well as genetic and metabolic diseases。There are 31 males and 22 females, and the average moon's age is 2.73±2.408。1.2 Control group:Forty-two non- cytomegalovirus infection infants who were synchronized admission。The depletion standard is not having the clinical symptoms such as jaundice and splenohepatomegalia and so on, CMV IgM negative, CMV-DNA in serum and urine are all less than 103copies/ml。There are 24 males and 18 females, and the average moon's age is 3.67±3.195。The differerce of gender and age between experimental group and control group is no significance。2. MethodsWe took infants' serum of femoral vein on an empty stomach, testing the quantity of exosome,the IL-4 and IFN-γlevels in serum and/or exosome as well as dipeptidyl peptidaseⅣ's activity by ELASA,then analyzed the difference between these two groups by SPSS11.5。Result1,The quantity of exosome in serum of experimental group is lower than the control group,and the difference has statistical significance。2,The level of IL-4 on exosome of experimental group is higher than the control group,and the difference has statistical significance。3,The level of IL-4 in serum of experimental group is higher,but the difference is not significant。On the other hand, the level of IFN-γin serum of experimental group is lower than the control group, and serum IFN-γ/IL-4 has the same tendency, both of them have statistical significance。4,Took all cases together, we found that the level of IL-4 in serum and on exosome have evident correlation (R=0.794), the correlation is significant in the experimental group (R=0.902)。5,The activity of dipeptidyl peptidaseⅣon exosome of experimental group is higher than control group, the difference has statistical significance。Conclusion1,After the infection of CMV, the immune balance of Th1 and Th2 cells has been broken, leading to the dominance of Th2 cells and immune suppression。2,The exosomes which have viral antigens are secreted less, so their fuction of angtigen presentation is weaken, and they can't stimulate bodise to remove the virus, therefor, chronic infection and immune tolerance appear。3,The secretion of cytokines appears abnormale, cytokines which related to Thl cells, such as IFN-γreduced, and the IL-4 which related to Th2 cells became rised。4,Dipeptidyl peptidase IV may participates the spreading and persistent infection of cytomegalovirus by means of exosome。... |
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