Panaxadiol Saponins Monomer And Its Combination With Other Herbs On The Inhibition Of Bacterial Biofilms

Pμrpose: Improve bacterial biofilm model of E.coli and Staphylococcus aureus, and establish sophisticated method of bacterial biofilm model; To Investigate influoence of antibacterial activity Chinese medicine monomer and ginsenoside on bacterial biofilm. Screen ginsenoside monomers which inhibits bacterial biofilm. Observe combined affect of ginsenoside and Chinese medicine monomer on bacterial biofilm.Method: Observe formation and morphology of bacterial biofilm with SEM and LSCM. With E.coli and Staphylococcus aureus as screening model of bacterial biofilm, coculture of bacterial biofilm with Chinese medicine monomers, ginsenosides and single ginsenoside, and measure growth capacity of bacteria and biofilm based on OD from microplate reader. Observe inhibitory capacity of different samples on bacteria and biofilms through inhibition rate. Screen other Chinese medicine monomers which have combined effect on bacterial biofilm with ginsenoside by broth microdilution.Resμlt: With the application of SEM and CLSM, it is observed that biofilm of E.coli and Staphylococcus aureus bacterial biofilm formation after 24 hours'Ginsenosides have strong inhibitory activity on E.coliu and biofilms, inhibition is 87.70% for E.coliu,and 69.65% for E.coliu biofilms, while they only inhibit Staphylococcus aureus and biofilms to some extent. Rb1,Rb2,Rb3,Rc,Rd, which inhibit E.coliu and biofilms greatly, were obtained after screening of ginsenoside monomers Rc's inhibition is 86.68% for E.coliu,and Rd's inhibition is 60.59% for E.coliu biofilms Combined medication shows, combination of Rb2 and Ferulic acid have collaborative inhibition on E.coliu and biofilms.Conclμsion: With application of SEM and CLSM, bacterial biofilm model is further improved. Bacterial biofilm model as screening model of chronic infection drugs is furthrt confirmed through investigation of impact of Chinese medicine monomers on bacterial and BBF. Ginsensides which inhibit bacterial and BBF greatly were obtained. In addition, ginsenoside which have collaborative effect with Ferulic acid was also obtained, which lays good foundation for further combined medicate with ginseng and treatment of chronic infection disease.