| Purpose: The main subject of observation of diabetes on high fat diet xiaokezhiping capsule with streptozotocin-induced diabetic rats lipid metabolism and adipose tissue MCP-1 expression levels, to observe the efficacy of the role of science for clinical medicine to provide Basis.Methods: In this experiment, Wistar rats fed high fat diet for 4 weeks, fasting can not help but water 12h, intraperitoneal injection of 2% of the streptozotocin 30mg/kg-1, the normal group were injected with the 0.1mol.L-1 sterile Citrate buffer. 72h when the eyes of all rats in blood serum was separated, -20℃cryopreservation of spare refrigerator, tested FPG, TG, TC, HDL, LDL,CRP. The successful model of the rats were randomly divided into model group, Xiaokezhiping capsule group, rosiglitazone maleate, xiaokezhiping capsule group were 3.6g/kg.d, rosiglitazone maleate Group fed by 4mg/kg.d the normal group and model group were given equal volume of distilled water. After 8 weeks with immunohistochemistry, protein levels were observed from the adipose tissue expression of MCP-1 situation.Results: 1,xiaokezhiping capsule can reduce the fat capsule in rats FPG, TG, TC and CRP levels, reduce body weight in diabetic rats. 2,diabetic rat adipose tissue expression of MCP-1 protein:①immunohistochemical results showed: normal adipose tissue expression of MCP-1 is weak, the range is small; model group, MCP-1 expression in adipose tissue significantly increased; Malaysia Acid in adipose tissue of rosiglitazone group compared with MCP-1 expression in model group decreased; xiaokezhiping capsule group MCP-1 expression in adipose tissue compared with model group decreased, the degree of expression of MCP-1 and rosiglitazone maleate close .②Semi-quantitative analysis showed: normal local weak staining, diffuse staining increased in model group, rosiglitazone maleate Strong Tendency of local coloring, xiaokezhiping capsule set of local weak staining was significantly lower than model group.Conclusion: 1,Xiaokezhiping capsule with glucose, lipid, reduce weight and improve the role of inflammation. 2, diabetic rat adipose tissue expression of MCP-1 protein levels, suggesting that MCP-1 may promote the development of obesity, T2DM important inflammatory factors; diabetes can reduce xiaokezhiping capsule in the diabetic rat adipose tissue MCP-1 protein Expression, suggesting that MCP-1 is one of the targets of the drug. |
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