The Capture Of Stasis Treatment Of Hemorrhagic Stroke And The Mechanisms Research

ObjectiveOrder summary history of primary physicians etiology and pathogenesis of hemorrhagic stroke, the discussion to explain Didangtang treatment of hemorrhagic stroke based on arguments Didangtang treatment of hemorrhagic stroke, the rational and scientific. And in previous studies of cerebral hemorrhage model, based on the combination of acute hemorrhagic stroke characteristics of the pathogenesis, the development of hypertensive cerebral blood stasis and heat of a model from the behavioral syndromes associated targets, the angle of the micro indicators to evaluate the model; and observe Didangtang the model, and to explore Didangtang treatment of acute hypertensive cerebral blood stasis and heat of a possible mechanism for the capture of stasis treatment of hypertensive cerebral blood stasis and heat of a experimental basis.MethodThe study is divided into two parts, theoretical and experimental study.Theoretical part:through access to ancient literature, reference and discussion of today's physicians issued publications, summary of past and present physicians finishing stroke prevention and treatment of bleeding point, and describes the capture of stasis treatment of hemorrhagic stroke basis.Experimental:The modified two kidney one clip renal hypertension in rats was established by the model. After a successful replication in the hypertension model, intraperitoneal injection of endotoxin (LPS), stereotaxic injection of collagenase, copy intracerebral hemorrhage rat model. Then were applied Didangtang its disassembled prescriptions peach rhubarb, leech fly insect treatment week. General situation of rats, blood pressure, neurological score (Bederson, LongaEZ, balance test), pathological changes, serum superoxide dismutase (SOD) malondialdehyde (MDA), cerebral hematoma surrounding tissue Na+-K+-ATP enzyme, interleukin -8 (IL-8)., protein C, nerve cell adhesion molecule -1 (ICAM-1), prothrombinase-2 (FGL-2), heme oxygenase-1 (HO-1), Angiopoietin -1 (Ang-1) levels.SPSS17.0 statistical package used for data statistical methods, data x-±s that the measurement data using single-factor analysis of variance between the two groups were compared using t test, compared among groups using analysis of variance F test. The homogeneity of variance with LSD-t method, missing variance with Dunnett-t method. Test level a of 0.05 (test of homogeneity of variance a is 0.10). P<0.05 was considered statistically different.ResultsTheory research:The effect of stasis with the capture of Didangtang treatment of hemorrhagic stroke is feasible and scientific, based on its use.Experimental study:Model and evaluation:General:rats appear to reduce dietary compounds, do not like moving, conjunctival congestion, ataxia, limb weakness or paralysis, even death and other symptoms; the spirit of sham-operated rats, activity, diet, no significant changes. BP:blood pressure rats, compared with the sham group, a statistically significant difference (P<0.05).Neurological score 1d:Bederson score:rats score about 2.22 points, the sham group score of 0, two groups, a statistically significant difference (P<0.05). LongaEZ score:score in model group at about 1.72, the sham group score 0 points, two groups, a statistically significant difference (P<0.05). Equilibrium test:the model group score at 4.28 minutes, score in the sham group at about 1.71, compared the two groups, a statistically significant difference (P <0.05).Pathology observed:light microscope model a large number of red blood cells were observed in bleeding area leakage, staining shallow, brain tissue shows red blood cells, inflammatory cell infiltration and brain cell gap becomes larger, loose tissue around the hematoma, softening, necrosis or even disappear, staining lighter, more red blood cells, leukocytes, and brain cells can see the gap bigger, cell swelling, large hematoma seen around the hemosiderin deposition; sham tissue space more closely, red blood cell distribution, no significant inflammatory cell infiltration.Biochemical tests:model group decreased SOD activity and MDA content increased, Na +-K +-ATP enzyme activity was reduced, compared with the sham group, were statistically significant (P<0.05)Immunohistochemistry:IL-8, ICAM-1, FGL-2, the model group were more positive cells appeared, the sham group, only a few positive cells,compared the two groups, a statistically significant difference (all P<0.05); protein C,model group, there were fewer positive cells, false surgery group more positive cells, compared the two groups, a statistically significant difference (all P<0.05).Western blot determination:HO-1, Ang-1 expression, decreased expression of the model group, compared with the sham group, a statistically significant difference (P<0.05).Didangtang and split the Treatment:Blood pressure:treatment groups, compared with model group, no significant difference (P>0.05).Neurological score 7d:Bederson score:Full score square in rats at about 1.54, rhubarb peach group at about 1.42, compared with model group, a statistically significant difference (P <0.05). LongaEZ Rating:rhubarb peach group score at 1.08 minutes, compared with model group, a statistically significant difference (P<0.05). Equilibrium test:rhubarb peach group scored 3.67 points or so, compared with model group, a statistically significant difference (P <0.05).Pathological observation:the whole side group of brain cells bigger space, bleeding areas of hemosiderin deposition, red blood cells, white blood cell infiltration; rhubarb peach group bleeding area more hemosiderin deposition, red blood cells, white blood cell infiltration, the more brain tissue cell gap the whole side group becomes large; leech bleeding area equivalent insects.groups scattered hemosiderin deposition, and less red blood cells, leukocytes, brain cells become larger gap, but smaller than the whole side group.Biochemical tests:SOD activity in all treatment groups, Na +-K +-ATP enzyme levels increased and MDA content decreased, compared with model group, treatment groups were statistically significant (P<0.05)Immunohistochemistry:IL-8, the treatment group decreased expression of positive cells, compared with model group, a statistically significant difference (P<0.05).ICAM-1, decreased expression of the whole side group, increased expression of leech fly insect groups, two groups compared with model group, a statistically significant difference (P<0.05). Rhubarb peach group, compared with model group, no significant difference (P> 0.05). Protein C, the increased expression of positive cells in the treatment group, compared with model group, a statistically significant difference (P<0.05). FGL-2, the treatment group decreased expression of positive cells, compared with model group, a statistically significant difference (P<0.05).Western blot determination:HO-1, increased expression of the whole side group, compared with model group, a statistically significant difference (P<0.05). Rhubarb peach group, leech fly insects compared with the model group, no significant difference (P>0.05). Ang-1 expression, whole-group, increased expression of rhubarb peach group, compared with model group, a statistically significant difference (P<0.05), leeches, insects fly compared with the model group, no significant difference (P> 0.05).ConclusionUsing Didangtang treatment of hemorrhagic stroke, theoretical, clinical, experimental basis, can be used to treat hemorrhagic stroke.Renal artery stenosis using ligation experiments, intraperitoneal injection of endotoxin LPS, intracerebral injection of collagenase hypertensive rat brain multi-factor out of stasis-heat model that the formation of cerebral hemorrhage and clinical development process close to the histopathological observation, neurological evaluation, biochemical and immunohistochemical indicators of test results show that the model can simulate some of the characteristics of hypertensive intracerebral hemorrhage, the use of brain-dimensional positioning technology, endotoxin, collagenase intracerebral injection method can be successfully produced multi-factor complex in rats the heat of hypertensive cerebral blood stasis model.The results show that Didangtang and its Ingredients on the stasis-heat syndrome with hypertensive intracerebral treatment effect may be through increased SOD content, Na +-K +-ATP enzyme activity and lower MDA content, reduce free radical oxidation and reduction reactions on the body injury, improve brain energy supply; inhibition FGL-2 expression, and promote protein C expression, to improve blood coagulation and anticoagulation system disorder; reduced IL-8, ICAM-1 and other inflammatory mediators, release, reduce or interrupt inflammation chain reaction; promote HO-1, Ang-1 expression, cut off the heme iron mediated oxidative stress response, promote angiogenesis, to reduce apoptosis, neuronal damage, cerebral edema, protecting nerve cells to treat brain The purpose of bleeding. The results suggest that arrived when the decoction and its Treatment of cerebral hemorrhage is the role of targets through multi-channel multi-role, and its possible mechanism by inhibiting the inflammatory response, antioxidant free radical damage, improve brain blood coagulation and anticoagulation system disorders, promote angiogenesis and to achieve the purpose of treatment of hemorrhagic stroke, however the specific mechanism needs further study.