Artemisia Decoction Of Additive Volume Control In The Treatment Of Chronic Non-bacterial Prostatitis Study

Purpose:The study is designed to observe the Artemisia decoction of additive volume control (Liu Siphonostegia, wild chrysanthemum, litchi) to rat model of CNP determination of serum TNF-α(ELISA method), iNOS in situ hybridization, the pathological changes of rat prostate;the purpose of the observation is to verify, to verify the Artemisia decoction of additive volume control of the efficacy of CNP, and to explore its mechanism of action, as Artemisia decoction of additive volume control provide a better experimental basis for clinical application.Process:We devided randomly 48 rats,male,weight,into 4 groups,12 rats in normal group (as groupⅠ), and the rest 36 rats were modeling. Successful model of the 36 rats were randomly divided into 3 groups,12 rats in the pathological group (as groupⅡ),12 rats in the Qiannietai group (as groupⅢ),and 12 rats in the Artemisia decoction of additive volume control (as groupⅣ). Rats are given appropriate medication;observed the general condition of each group (including its condition of survival and its body weight). After 4 weeks, abdominal aortic blood,testing blood TNF-α(ELISA method);executed and take its prostate tissue, to detect the average wet weight of prostate tissue and the ratio of prostate wet weight/body weight. Some we used the conventional microscope's light to observe the pathological changes of the prostate of the rats,it either real or interstitial;another part of the prostate for the detection of iNOS in situ hybridization results with the pathological image analysis system.Results:(1) In clinical model, the content of TNF-αis comprehensively higher than the blank group(p<0.01); in the Artemisia decoction of additive volume control and Qiannietai group, there is comprehensive difference in the content of TNF-α,(P<0.01);as for the statistics fields, the Artemisia decoction of additive volume control is more significant than the Qiannietai group.(2) clinical model iNOS has a better influnce than the blank comparison group (P<0.01);there is comprehensive difference among the Artemisia decoction of additive volume control,Qiannietai group and pathological group groups.(3) Artemisia decoction of additive volume control has great positive influence in the rats prostate tissue. Conclusion:Through this study I found out that:(1) Artemisia decoction of additive volume control can be effective in treating CNP;(2) Artemisia decoction of additive volume control treatment of CNP mechanism, possibly achieved by reducing the inhibition of TNF-αcontent and iNOS prostate tissue.