| ObjectivesCerebral ischemia reperfusion injury (CIRI) is the phenomenon in which the injury of the tissues and the organs was getting more and more serious after being reperfused on the basic of ischemia.It is generally accepted that IRI is a process from a reversible injury (ischemia injury) to an inreversible injury (reperfusion injury). The pathogenesis of ischemia reperfusion injury involves calcium overload, generation of oxygen free radicals,the activation of the apoptosis gene and the disorder of mitochondria function.Oxygen free radicals are considered to be one of important factors involved in the pathophysiology of ischemia-reperfusion injury.Cordyceps sinensis is a kind of rare medicinal fungi with multiple pharmacological effects of improving immunity, anti-tumor,anti-oxidation,Free radical scavenging, anti-organ fibrosis, antiarrhythmic, reducing the blood pressure,e xpanding bronchial, preventing pulmonary edema and regulating the liver and kidney function.The aim of the experiment is to establish brain cerebral ischemia/reperfusion injury model, observe the therapeutic effect of the aqueous extract of Cordyceps sinensis (CS) on cerebral ischemia/reperfusion injur and explore its mechanism in rat model, and lay a theoretical and experimental basis for the application and further development of CS.Methods60 rats were divided randomly into five groups:Sham-operated group (n=12), cerebral ischemia-reperfusion model group (n=12), CS treated group I (n=12), CS treated group II (n=12), and CS treated group III (n=12). Cerebral ischemia-reperfusion model was induced by inserting the thread into middle cerebralartery for 2 hours. After the ischemia occurred, same dosage of phosphate buffer solution (PBS) were administered to the rats of sham group and cerebral ischemia-reperfusion group via intraperitoneal injection(ip) respectively each day.150mg/kg,300mg/kg, 600mg/kg of CS were administered to the rats of CS I, CSⅡand CSⅢgroup via intraperitoneal injection (ip) respectively each day. Seven days later, neurological defective scores, brain water content and malondialdehyde (MDA) content, activity of superoxide dismutase (SOD), activity of GSH-PX, and activity of CAT were measured respectively.Results1. All the CS treatde groups recovered better than cerebral ischemia-reperfusion group (P＜0.05);2. The brain water contents of the CS treatde groups were significantly lower than those of ischemia-reperfusion group (P＜0.05).3. CS may significantly suppressed MDA content, prevented the reduction of SOD activity, GSH-PX activity and CAT activity in comparison with the ischemia-reperfusion group (P<0.01).ConclusionCS may had an apparent protection against cerebral ischemia reperfusion injury in rats, the machenism of which may be related to preveting the reduction of SOD activity, GSH-PX activity and CAT activity, and the inhibion of the oxidative free radical production after ischemia-reperfusion injury. |
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