Recombinant Human Endostatin Combined With Radiation On Ovarian Cancer Cells H0-8910

Ovarian cancer is the one of the most serious life-threatening tumor to the female,and the the mortality rate ranks first in gynecologic tumors. At present, the main therapies of ovarian cancer are mostly surgical operation, integrated with chemical therapy, or radiotherapy. The high invasion ability and the histological differentiation of the ovarian cancer are different in the radiosensitivity. Therefore, the ideal radiaosensitizer is the focus of the research.ObjectiveIn vitro effects of recombinant human endostatin alone or combined with radiation effects on human ovarian cancer HO-8910, to observe whether the radiosensitization occurred, and to explore the mechanism of radiosensitization.MethodsThe ovarian cancer HO-8910 cells were exposed to different concentrations of human endostatin, MTT experiments were done to obtain the cell proliferation inhibition rate and IC20, cell colony formation assay were done to observe the radiosensitization of recombinant human endostatin on ovarian cancer HO-8910 cells.Cell survival curves were drawn to calculate the sensitization enhancement ratio (SER). Flow cytometry were done to analyze the effects on cell apoptosis of drug and radiation alone and combined group. Results1.MTT experimental results showed that the cell inhibition rate were 0.0. 0.73%.9.39%,20.63%,30.89%,35.31%.41.15%, after the effects of different concentrations (5.25.50.100,250.500.750.1 OOOmg/L)on ovarian cancer HO-8910 cells for 24 hours, and the clear dose-response relationship was that the IC20 of ovarian cancer cells was 222.06 mg/L.2. The results of cell colony formation assay showed that:100 mg/L-200 mg/L concentration of the drug combination with radiation group were lower than the Do. Dq values of the control group, the sensitization enhancement ratios were 1.16 and 1.26, respectively.3. The results of flow cytometry showed:As the radiation dose increases, the apoptosis rate increased significantly; addtionnaly, when drugs of different concentrations combined with the same dose of radiation, the apoptotic rate was higher than the radiation group alone, and apoptosis rate increased along with the increase of drug's concentration. When endostatin effected on the cells, phase GO + G1 of the cells prolonged, and the radiation caused the blockage of phase G2+M significantly.Conclusions1. The cytotoxicity effects of recombinant human endostatin on ovarian cancer HO-8910 cells were significant and it depended on the time and concentration. The possible mechanism may be that it directly inhibited the proliferation and migration of the tumor cells.2. Low-dose recombinant human endostatin improved the radiosensitivity of ovarian cancer HO-8910 cells and the mechanism may be that it induced the apoptosis of tumor cells while it increased the oxygen content of tumor cells, then it played a role in radiosensitization.3. Recombinant human endostatin may become the new treatment for cancer radiation sensitizer.