| BackgroundMatrix metalloproteinases are a class of highly conserved proteolytic enzymes, which can disintegrate the extracellular matrix and promote tumor angiogenesis, so that enhance invasion and metastasis of the cancer cells. MMPs can be divided into five classes, in which MMP-2 and MMP-9 have close relations with the invasion and metastasis of tumor.RECK gene is a tumor suppressor gene, can restrain the invasion and metastasis of tumor through inhibiting the expression of MMPs.UP to now, abnormal expression of RECK, MMP-2 and MMP-9 have been found in many carcinomas such as primary hepatocellular carcinoma, gastric cancer, colorectal cancer, cervical carcinoma, lung cancer and so on. Clear cell renal cell carcinoma (CCRCC) is familiar in urinary system, but the research about the expression of RECK, MMP-2 and MMP-9 in CCRCC and their relationship is scarce.ObjectiveResearch the expression of RECK, MMP-2 and MMP-9 in CCRCC tissue and normal renal tissue, explore their effects on the occurrence and development of CCRCC and their relationship. MethodImmunohistochemistry was used to detect the expression of RECK, MMP-2 and MMP-9 in 40 cases of CCRCC tissue and 20 cases of normal renal tissue.Results1.The positive expression rate of RECK in CCRCC tissue is 55%, while 85% in normal renal tissue, there is significant difference between the two(χ2=5.275, P=0.022). The positive expression rate of RECK in CCRCC tissue of the patients whose age less than 60 is 70%, while 52% in age exceeding or equal 60, there is no significant difference between the two(χ2=0.175, P=0.676). The positive expression rate of RECK in CCRCC of male patients is 48%, while 67% in female patients, there is no significant difference between the two(χ2=1.320, P=0.251). The positive expression rate of RECK in CCRCC tissue of Fuhrman pathological grading 1 and 2 is 71%, while 31% in Fuhrman pathological grading 3 and 4, there is significant difference between the two(χ2=6.077, P=0.014). The positive expression rate of RECK in CCRCC tissue of clinical stageⅠandⅡis 67%, while 20% in clinical stageⅢandⅣ, there is significant difference between the two(χ2=6.599, P=0.010).2. The positive expression rate of MMP-2 in CCRCC tissue was 73%, while 30 % in normal renal tissue, there was significant difference between the two(χ2=9.909, P=0.002). The positive expression rate of MMP-2 in CCRCC tissue of the patients whose age less than 60 was 82%, while 65% in age exceeding or equal 60, there was no significant difference between the two(χ2=1.440, P=0.230). The positive expression rate of MMP-2 in CCRCC tissue of male patients was 80%,while 60% in female patients, there was no significant difference between the two(χ2=1.881, P=0.170). The positive expression rate of MMP-2 in CCRCC tissue of Fuhrman pathological grading 1 and 2 was 58%, while 94% in Fuhrman pathological grading 3 and 4, there was significant difference between the two(χ2=6.040, P=0.014). The positive expression rate of MMP-2 in CCRCC tissue of clinical stageⅠandⅡwas 70 %,while 80% in clinical stageⅢandⅣ, there was no significant difference between the two(χ2=0.376, P=0.540).3. The positive expression rate of MMP-9 in CCRCC tissue was 78%, while 25 %in normal renal tissue, there was significant difference between the two(χ2=15.314, P=0.000). The positive expression rate of MMP-9 in CCRCC tissue of the patients whose age less than 60 was 82%, while 74% in age exceeding or equal 60, there was no significant difference between the two(χ2=0.399,P=0.527). The positive expression rate of MMP-9 in CCRCC tissue of male patients was 80%,while 73% in female patients, there was no significant difference between the two(χ2=0.239, P=0.625). The positive expression rate of MMP-9 in CCRCC tissue of Fuhrman pathological grading 1 and 2 was 67%, while 94% in Fuhrman pathological grading 3 and 4,there was significant difference between the two(χ2=4.038, P=0.044). The positive expression rate of MMP-9 in CCRCC tissue of clinical stageⅠandⅡwas 73 %, while 90% in clinical stageⅢandⅣ, there was no significant difference between the two(χ2=1.195, P=0.274).4. In CCRCC tissue, the expression of RECK and MMP-2 was negatively correlated (r=-0.572, P<0.05). In CCRCC tissue, the expression of RECK and MMP-9 was negatively correlated (r=-0.649, P<0.05).Conclusion1. The expression of RECK in CCRCC tissue was low, and its low expression was related to the pathological grading and clinical stage of the tumor, suggesting that RECK can restrain the occurrence and progression, may be a useful marker in diagnosis and prognosis of renal clear cell carcinoma.2. MMP-2 and MMP-9 were highly expressed in CCRCC, and their high expressions were related to the pathological grading, suggesting that MMP-2 and MMP-9 can promote the occurrence and progression, may be useful markers in diagnosis and prognosis of renal clear cell carcinoma.3. The expression of RECK in CCRCC was negatively correlated with MMP-2 and MMP-9, suggesting that RECK and MMP-2, MMP-9 were interrelated in the occurrence and progression of CCRCC, and the inhibitory effect of RECK on CCRCC may work through its inhibition on MMP-2 and MMP-9. |
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