The Correlation Between Ppar-γ And VEGF Expression In The Lung Tissue Of Congenital Heart Disease

ObjectiveTo investigate the relationship between the expression of PPAR-G mRNA and VEGF mRNA and the expression in the lung tissue of Congenital heart disease with pulmonary hypertensionMethods30 patients underwent ventricular septal defect repaired were divided into 3groups according to the mPAP measured during operation:groupⅠwith no PAH(mPAP< 20mmhg,n== 12),groupⅡwith slight and moderate PAH(21<mPAP< 50mmhg,n=10), groupⅢwith severe PAH(mPAP>50mmhg, n=8)Quantitative reverse-transcription polymerase chain reaction (RT-PCR) was used to examine the levels of PPAR-γmRNA and VEGF mRNA in the 3 groupsResultThe relative amount of PPAR-γmRNA and VEGF mRNA expressed in groupⅠⅡⅡwere 0.583±0.080,0.254±0.024,0.157±0.025,and 0.709±0.071,0.339±0.033,0.211±0.043,respectively.The expression of these two genes in groupⅠⅡwas significantly higher than in groupⅢ(P<0.001). The relative amount of PPAR-G mRNA and VEGF mRNA in groupⅠwas significantly greater than in groupⅡ(P<0.01).PPAR-γexpression was positively correlated with VEGF expressionConclusionHigh PPAR-γand VEGF expression levels are detected in the lung tissue of Congenital heart disease with pulmonary hypertension. These two genes play an essential role in tumor development and growth. PPAR-γmay stimulate angiogenesis through VEGF up-regulation...