The Study Of The Antimicrobial Activity Of The Ventriculoperitoneal Shunt System Treated By PVP-I

Objective: To compare the antimicrobial activity of the tube of ventriculoperitoneal shunt treated by povidone-iodine(PVP-I) and that of not treated by povidone-iodine.Methods: We built a in vitro ventriculoperitoneal shunt system, and two bacteria, Staphylococcus epidermidis group (A group) and the E. coli group (B group), were selected for the experiment. Various amounts of 0.1% PVP-I were injected into certain concentrations of bacteria. And the bacteriological features of the effluents and tube of the shunt were observed and recorded. We used the simulator system to simulate the fluid from the ventricle through the shunt system into the abdominal cavity client side, and controlled the flow rate by the intravenous pumps. Also we had a different order on the injection of different liquids (the order of a: bacilliâ†'salineâ†'povidone-iodine, the order of b : povidone-iodineâ†'bacilliâ†'saline). And we took the bacteriological examination of the effluent of the simulator and the tubes of the shunt.Results: 0.1ml 0.1% PVP-I could kill the bacteria of different concentrations which contained 5ml bacilli with 488cfu / 10μl of S. epidermidis and 5ml bacilli with 455 cfu/ 10μl of E. coli completely. The effluent of the simulator without PVP-I treatment were visible bacterial growth (A: 57 cfu / 10μl; B: 239 cfu / 10μl), and its walls were were visible bacterial growth, too. On the other side, effluents with the treatment and the flush of the flow were nearly sterile, so were its wall. The access point of the simulator system showed a reduction in the number of bacteria after PVP-I treatment.Conclusion: With PVP-I wash and filling, the chance of bacteria growth inside the system can be reduced.