Effect Of Tetrandrine To The Multidrug Resistance And Apoptosis Of Pancreatic Cancer Cell

Pancreatic cancer is one of common malignant tumors of the digestive system, early difficult to find, usually diagnosed at advanced stage. Currently 85% of pancreatic cancer patients died within 1 year after diagnosis,5-year survival rate of less than 5%. Most patients have attacks due to treatment or transfer to the pancreas and other organs can not be removed, surgery was only about 20%. Currently, adjuvant therapy of pancreatic cancer is disappointing, at present its main treatment is chemotherapy, but most of the poor drug efficacy, side effects, efficacy of chemotherapy drugs is relatively good response rate of 25% of its clinical application of gemcitabine chemotherapy is only 28% efficient, and chemotherapy can not improve the outcome. Multidrug resistance (Multi-drug resistance, MDR) of pancreatic cancer chemotherapy have led to failure of the main reasons. Studies have shown that anti-Chinese Han alkaloids have been extracted factors tetrandrine significantly inhibited pancreatic cancer. This study further clarify the elements of tetrandrine and apoptosis resistance of pancreatic cancer drug resistance mechanisms, a better understanding of tetrandrine on the resistance to apoptosis regulatory factors for the future opening of tetrandrine Reversal of provision of new means of resistanceObjective To explore the effect of Tetrandrine to the multidrug resistance and apoptosis of pancreatic cancer cell.Methods Human pancreatic cancer cell SW1990 which is sensitive to Gemcitabine and SW1990/Gem which is resistant to Gemcitabine were slected and divided into 3 groups, the control group,Gemcitabine group and Gemcitabine with Tetrandrine group. The cytotoxic effect of anticancer drugs was examined by MTT methods and apoptosis of pancreatic cancer cell was examined by PI coloration.Results The RF of SW1990/Gem group which is resistant to Gemcitabine was 4.70, Meanwhile in Gemcitabine with Tetrandrine group, it descended to 1.69. This revealed that Tetrandrine have reversal effect on multidrug resistance in Human pancreatic cancer cell SW1990/Gem. On 12h,18h,24h, we examined the apoptosis of Tet/GEM group,GEM group and control group, the difference was distinct. This revealed that Tetrandrine with Gemcitabine can help to produce apoptosis in multidrug resistance human pancreatic cancer cell.Conclusion The combined application of Western medicine, significant improvement in clinical remission of pancreatic cancer and survival. Chinese Traditional Medicine and Chinese Herbal Medicine in the sensitizing effect of clinical and experimental studies in has been fully confirmed, but the exact mechanism yet to be confirmed. Tetrandrine element is Menispermaceae Stephania tetrandra species isolated from the major tuber alkaloids, and its chemical structure is a two-benzyl isoquinoline in the cell membrane transport and distribution use. Han has been a factor on anti-pancreatic cancer, esophageal cancer and breast cancer and other solid tumors and leukemia have certain direct inhibitory effect. Animal experiments show that tetrandrine hormone is an effective radiation sensitizer, hypoxia cancer cells by improving the state of sensitizing effect of the sky. Natural medicine tetrandrine prime addition to having the tumor cells by increasing concentrations of chemotherapy drugs reverse the pancreatic cancer cell line SW1990-GEM multidrug resistance, to promote the chemotherapy drug-induced apoptosis of tumor cells, but also can inhibit tumor cell proliferation and with promoting their apoptosis, suggesting that patients with advanced cancer clinical anticancer drugs can be used in combination with its natural active ingredients enhance the therapeutic effect. This study shows that by the prime role of tetrandrine pancreatic cancer cells resistant strains after the RF down 4.70 from the 1.69 before the treatment, indicating that tetrandrine hormone can reverse SW1990/Gem resistant pancreatic cancer cell resistance to gemcitabine drug, its effect may be associated with chemoresistance in pancreatic cancer cells reduced the expression of MRP protein. Also, direct activation of gemcitabine MRP gene to the transcription level of expression of MRP gene, known as collaborative tetrandrine on gemcitabine chemoresistance in pancreatic cancer cells enhanced apoptosis. Therefore, further into the details of the pancreatic cancer cells of multiple mechanisms, sensitive to pancreatic cancer cells to find and rebuild the apoptosis pathway and reverse the anti-apoptotic state, for the completely overcome primary drug resistance of pancreatic cancer has important significance.