Association Of Genetic Polymorphism Of Prostate Specific Antigen With Prostate Cancer Risk

Objective:A Single nucleotide polymorphism with adenine (A) to guanine(G) substitution is identified at position-158 in the androgen response element region of the prostate specific antigen(PSA)gene. We evaluated the relationship between the PSA-158A/G polymorphism and the risk, clinic stage and pathologic grade of prostate cancer.Materials and Methods:peripheral venous blood samples were obtained from 162 patients with prostate cancer and 180 controls with benign prostatic hyperplasia. The diagnosis, tumor volume and pathological stage of prostate cancer were alldetermined according to the pathological reports of transrectal ultrasound guided prostate biopsy, transurethral prostate resection and radical prostatectomy. The PSA-158A/G polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism methods.Results:The case and controls did not differ significantly with respect to age and prostate volume. Mean age of PCa subject was 65 year and 60 years for control group,the mean serum PSA levels, measured at time of diagnosis, was averaged 30.8 ng/ml in prostate caner patients and 4.7ng/ml in controls (p<0.001).Patients with prostate cancer there was a significantly greater frequency of the GG genotype (68.5%vs 62.2%) than that in the control group (p= 0.028). Relative to GG genotype, The OR of GG to AG and AA was(0.42,95%,CL:0.17-0.11;P=0.04) and (1.27,95%, CI 0.75-2.14;P=0.359).Furthermore, GG genotype also is more frequent in advanced prostate cancer than GA and AA genotype(OR 2.17,95%;CL:0.107-4.410;P=0.029)Conclusions:The PSA -158A/G polymorphism is associated with prostate cancer. The GG genotype possibly decreases the risk of prostate cancer and the GG genotype is associated with advanced clinic stages...