Expression Of Vascular Endothelial Growth Factor B And Its Isoforms In Hepatocellular Carcinoma And Its Clinical Significance

Hepatocellular carcinoma is one of the most common malignancies, its incidence and death rate related in cancer are high, and it is a serious threat to human health, and therefore,prevention for HCC become a important issue for cancer research. Tumor angiogenesis throughout the occurrence, development and transfer process, is a complicated process, is regulated by many kinds of internal and external factor. HCC is a typical kind oftumor with rich blood, and more vascular is a important features of HCC.so,angiogenesis has become a hot issue in the study of liver cancer currently. Vascular endothelial growth factor (vascular endothelial cell factor, VEGF) have closely related to the formation of blood vessels of HCC, the family includes: VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PLGF and VEGF-F 7 members and their respective isoforms. VEGF-A is acknowledged to be the strongest one in promoting factor, its role in the process of angiogenesis in HCC have studied by many people from many countries all over the world. VEGF-B is another member of the family, experiment has proved that VEGF-B band to VEGFR-1 and promote angiogenesis. HCC is a typical of more vascular tumor, to find the role of VEGF-B and its isoforms in HCC,our experiment using real-time quantitative PCR testing VEGF-B, VEGF-B167 and VEGF-B186 mRNA in HCC tissue and assess its clinical significance.AIM:Detect the mRNA expression of VEGF-B and its isoform VEGF-B 167, VEGF-B186 in liver tissue and assess its clinical significance.METHODS:We Collect 98 cases of tissue samples from HCC patients receive surgical resection:including 98 tumor tissue samples and related no cancer tissue beside the tumor. We also Collect 24 normal liver(NL),5 hepatitis(HV),32 liver cirrhosis(LC).Then we examined the mRNA expression of VEGF-B and its two isoform VEGF-B167, VEGF-B186 in liver cancer, non-cancerous tissues, liver cirrhosis, chronic hepatitis B and normal liver tissues using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) analysis, analysis the correlation with clinical pathological data of patients, and do survival analysis with follow-up data information. RESULTS:Total VEGF-B and VEGF-b186 have no statistically significant between each group, and the expression level of VEGF-B167 have statistically significant between each group, (P=0.005), LC group minimum, median 0.440, normal group has a highest median of 1.368; The expression level of VEGF-B167 in HCC group (1.312) is obviously higher than that in HV group (0.526) and LC group (0.440), and the expression level increase from N3 group to T group. The samples in HCC group with portal venous tumor bolt have higher level VEGF-B167 then those without portal venous tumor bolt. The group with transfer have higher level VEGF-B167 then the group without transfer. According to the expression level of VEGF-B, patients with HCC were devided into three groups, the overall survival rates of low expression VEGF-B167 group tended to be higher than high expression group and no difference group (P=0.097), the tumor-free survival rate of low expression VEGF-B167 was significantly higher than other groups (P=0.023); there was no significant correlation between total VEGF-B, VEGF-B167 and VEGF-B186 expression level and sex, age, virus infection, liver cirrhosis, Child-pugh score, AFP, tumor multiplicity, tumor size, vascular invasion, TNM stage and tumor differentiation. COX regression analysis found that Child-pugh classification (P= 0.001), tumor number (P=0.020), tumor diameter (P<0.001), vascular invasion (P= 0.003), TNM stage (P=0.047), tumor differentiation (P=0.043) are the risk factors affect survival time; And the Child-pugh grade (P=0.022), tumor number (P= anti-frost), tumor diameter (P=0.006), vascular invasion (P=0.019), TNM stage (P =0.025) are the risk factors affect tumor-free survival time. Did not find total VEGF-B, VEGF-B167, VEGF-B186 is risk factors affecting survival time and tumor-free survival time.CONCLUSION: VEGF-B involves in hepatocarcinogenesis and VEGF-B167 plays a more important role than VEGF-B186 in this process.High expression of VEGF-B167 promotes portal venous tumor formation and tumor metastasis. Patients with low expression of VEGF-B167 have good prognosis, their postoperative overall survival rates and tumor-free survival rates are higher.