Study On The Genetic Susceptibility Between KDR And Gliomas In Chinese Population

Objectives:our purpose was to determine whether the polymorphisms (rs2071559 and rs2239702) in KDR are associated with risk of gliomas in a Chinese population.Methods:in this study,we analyzed single nucleiotide polymorphism of KDR in 504 patients with gliomas and 530 gender-and age-matched controls.We collected blood samples and clinical materials,extracted DNA and performed genotyping with MassARRAY Sequenom SNP time-of-flight mass spectra chip system.Haploview version 4.1 was used to test Hardy-Weinberg equilibrum(HWE) and SPSS version 17.0 was used for single locus analysis.Results:the results of genotyping showed that the genotyping ratio of KDR-rs20 71559 and KDR-rs2239702 was 99.7% and 99.8%,respectively. HWE-P value of the two loci was 0.424 and 0.712,respectively.Both of them were larger than 0.05,which suggested that the allele frequency of the controls was in balance.The analysis of allele showed that C allele of KDR-rs2071559 and A allele of KDR-rs2239702 were both risk allele of gliomas.Both of them can increase the risk of gliomas (OR=1.424, 95%CI:1.186-1.710,P=0.000 and OR=1.349,95%CI:1.070-1.700, P=0.011,respective-ly).The analysis of genotype suggested that individuals with KDR-rs2071559 C/T or C/C genotype showed increased glioma risk(adjusted OR**=1.942,95%CI:1.291-2.921, P=0.001 and adjusted OR**=1.405,95%CI:1.070-1.845, P=0.014, respective-ly)whereas those who carried KDR-rs2239702 A/A genotype showed increased glioma risk (adjusted OR**=2.387,95%CI:1.197-4.759, P=0.014). Stritified analysis showed that KDR-rs2071559 C allele, C/C and C/C genotype were all associated with higher risk for never-smoker, astrocytoma,other gliomas,gradeⅡand gradeⅢgliomas; KDR-rs2239702 A allele and A/A genotype were both associated with higher risk for never-smoker, other glioma and gradeⅠglioma.Meanwhile, KDR-rs2239702 A/A genotype was also associated with gradeⅡglioma.Analysis of cumulative effect showed that individuals carrying 1,3 and 4 risk alleles had a higher risk of developing gliomas compared with those who carried 0 risk allele (OR=1.534,95%CI=1.123-2.096,P=0.007;OR=1.960595%CI=1.163-3.304,P=0.011; OR=2.748,95%CI=1.383-5.462,P=0.003respectively), and glioma risk increased with increasing numbers of risk variant alleles.Conclusions:C allele of KDR-rs2071559 and A allele of KDR-rs2239702 was risk allele of giomas,respectively.They were both associated with increased glioma risk.,and the risk of developing gilomas of those who carried risk allele increased with increasing numbers of risk variant alleles.The variant genotype KDR-rs2071559 C/T or C/C were associated with a significantly increased risk of glioma,especially astrocytoma and other gliomas,when compared with wild-type homozygote T/T.And the variant genotype KDR-rs2239702 A/A was associated with a significantly increased risk of gliomas, especially other gliomas,when compared with wildtype homozygote G/G. MassARRAY Sequenom SNP time-of-flight mass spectra chip system is a good method for screening SNPs of gene with the excellence of speediness and high flux.