| Objective1. To investigate the changes of peripheral EPCs in rats with traumatic optic nerve injury, and analyze the correlations between the EPCs and leukocytes, platelets.2. To analyze the affect of EPCs in rats with traumatic optic nerve injury.3. To investigate the effect of EPCs on traumatic optic nerve injury of rats, which can provide the theoretical evidence for further therapy.Methods:1. The animal model of traumatic optic nerver injury was created by fluid percussion brain injury device (FPI), The rats were divided into control group (n=20) and traumatic optic nerve injury group (n=60) randomly. According to the crackdown, traumatic optic nerve injury group were divided into mild injury group (n=20), moderate injury group (n=20) and severe injury group (n=20). The number of EPCss leukocytes and platelets of 24h before injury and 3,12,24,48,72 h,1w,2w,3w after injury were observed every group.2. The animal model of traumatic optic nerver injury was created by fluid percussion brain injury device(FPI), The rats were divided into control group and traumatic optic nerve injury group randomly. Every group was divided into 9 subgroups(24 hours before injury and 3,12,24,48,72h,1,2,3w after injury) by the time of injury. The number of EPCs in peripheral blood,HE and CD31 immunohisto-chemistry markers vascular endothelial,flash-visual evoked potential(F-VEP) were used to check variations in traumatic optic nerve injury at every time point above. The correlation between different items were analyzed by Pearson test.Results:1. The number of EPCs were(46～52)/200000 monocytes in normal rats and reduc-ed to minimum at 3h after traumatic optic nerve injury. There was not a significant difference in all groups. The EPCs increased above normal level at 24～72h in mild and moderate injury group with traumatic optic nerve injury, and peak at 72h, then reduced to basic level gradually. The EPCs rised at 24～72h after traumatic optic nerve injury, but could not reach normal level in severe injury group. The changes of EPCs were not correlated with peripheral leukocytes and platelets (r=0.43,0.41,0.43,0.47; P＜0.01). 2. The number of CD31+cell were (7-9)/5 high power field in normal rats's optic nerve and surrounding tissue. After traumatic optic nerve injury, the number of CD31+cell were (8.36±1.52,7.17±1.1. 10.41±1.92,11.43±1.58,14.29±2.03,17.33±1.47,17.86±1.22,18.13±1.40)/5 high power field. Comparison of the traumatic optic nerve injury group and control group, there was a significant difference at 48h/72h,1w,2w,3w time point(t=4.31,-7.61,-8.17,-10.08,-10.79; P<0.05). The number of microvascular were(6～9)15 high power field in normal rats's optic nerve and surrounding tissue. After traumatic optic nerve injury, the number of microvascular were (6.52±1.05,7.54±2.0,8.52±2.2,11.02±1.62,15.40±2.04,18.39±1.96,23.21±1.50,22.78±2.40,24.13±2.51)/5 high power field. Comparison of the traumatic optic nerve injury group and control group, there was a significant difference at 48h,72h,1w,2w,3w time point (t=4.25,-7.74,-8.26,-10.28,-11.49; P＜0.05).The latency period of P waves was decreased at 3h and increased to above basic level at 24h, and then tend to stable. Comparison of the traumatic optic nerve injury group and control group, there was a significant difference at 3h,12h,24h,48h,72h,1w,2w,3w (t=4.15,3.74, 5.84,6.08,6.40,6.52,6.53,6.61; P＜0.05).The amplitude of F-VEP was decreased at 3h and increased to basic level at 12h, then decreased to below basic level gradually. Comparison of the traumatic optic nerve injury group and control group, there was a significant difference at 3h,24h,48h,72h,1w,2w,3w (t=3.95, 4.14,5.26,5.78,6.49,6.72,6.23;P<0.05).The number of EPCs has correlation with CD31+cell, microvascular and F-VEP.Conclusion:1. The number of EPCs in peripheral blood increased first and then reduced to normal level in rats with traumatic optic nerve injury. The more severe optic nerve injury was, the less increase extent of EPCs was.2. Traumatic optic nerve injury can stimulate the number increased of EPCs in peripheral blood.3. The EPCs can home to the traumatic area to repair injured tissue and enhance angiogenesis in rats with traumatic optic nerve injury. |
PDF Full Text Request