| Objective:This study was aimed to investigate the expression of tumour necrosis factor-α(TNF-α), and interleukin-10 (IL-10) in inflamed and non-flamed intestinal mucosa from patients with Crohn's disease, and explore its relationship with disease. To analyze the significance of TNF-α, IL-10 in occurrence, development and treatment with Crohn's disease.Methods:30 patients'postsurgical paraffin blocks in our hospital with CD confirmed by clinical and pathological were enrolled in the study. Respectively select the inflamed and normal paraffin blocks for experimental groups. Based on《The standard of diagnosis and treatment of IBD Consensus》2007, jinan,17 patients were classified into moderate groups,13 patients were classified into severe groups. meanwhile,30 patients'postsurgical paraffin blocks in our hospital with colon carcinoma were selected, to chose the no cancer tissue regarded as normal subjects for control groups. The expression of TNF-αand IL-10 in intestinal mucosa were determined by immunohistochemistry. Meanwhile, a rank correlation analysis between the strength of TNF-a expression,IL-10 expression and clinical stages is made.Results:1. Immunohistochemical staining analysis localized the expression of TNF-αto intestinal mononuclear cell inlamina propria. There were 21 TNF-αpositive cases in 30 inflamed intestinal mucosa of Crohn's disease (70%),19 in 30 non-flamed intestinal mucosa of Crohn's disease (63.33%),and 3 in 30 control group (10%). there was significant difference between inflamed intestinal mucosa of CD and control (P<0.05),and also non-flamed intestinal mucosa of CD and control (P<0.05).2. Immunohistochemical staining analysis localized the expression of IL-10 to intestinal epithelial cells and lamina propri. There were 21 IL-10 positive cases in 30 inflamed intestinal mucosa of CD (70%),14 in 30 non-flamed intestinal mucosa of CD (46.67%),and 13 in 30 control group (43.33%). there was significant difference between inflamed intestinal mucosa of CD and control (P<0.0125),but no significant difference between non-flamed intestinal mucosa of CD and control (P>0.0125).3. In inflamed intestinal mucosa from patients with CD, the level of TNF-a was higher in both moderate and severe groups than in controls (P0.0125), but no significant difference between moderate and severe groups. In inflamed intestinal mucosa, the level of TNF-αwas higher in severe groups than controls, but not in moderate and controls(P>0.0125), moderate and severe groups(P>0.0125). The strength of TNF-αexpression in inflamed and non-flamed intestinal mucosa from patients with CD were positively correlated with the severity of the disease(respectively r=0.609,P=0.001<0.05 and r=0.356, P=0.037<0.05).4. In inflamed intestinal mucosa from patients with CD, the level of IL-10 was higher in moderate groups than in controls (P<0.0125), but not in moderate groups and severe groups(P>0.0125),severe groups and controls(P>0.0125). In non-flamed intestinal mucosa from patients with CD, there were no significant difference between moderate groups and controls(P>0.0125),moderate groups and severe groups(P>0.0125),severe groups and controls(P>0.0125). The strength of IL-10 expression in inflamed and non-flamed intestinal mucosa from patients with CD were not correlated with the severity of the disease(respectively r=0.261, P=0.164 and r=0.128, P=0.499).Conclusions:1. TNF-αoverexpression both in inflamed and non-flamed intestinal mucosa from patients with CD, and positively correlated with the activity of CD. suggest that TNF-αplay a important role in the pathogenesis of CD. TNF-αlevel could reflect the pathogenetic condition. TNF-αcould be considered as a simple biological parameter for the clinic effect and prognosis of CD, could be a directions for the clinic therapeutics.2. IL-10 was considered as a anti-inflammation cytokine that overexpression in inflamed mucosa of CD,suggest that IL-10 play a important role in inhibiting acute inflammation course and controling the progress of pathogenetic condition in CD. |
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