| Arabidopsis magnesium transport family members (AtMGTs) play important roles in magnesium uptake and transport, and AtMGT4is a member among them. Our previous data displayed the increased seed size phenotype in AtMGT4overexpression transgenic plants(AtMGT4-ox). Seed size (thousand grain weight) is one of the important agronomic characters of the crops. It is of special importance to increase the crop yield and enhance the production safety by using genetic improvement approach to increase the thousand grain weight while without changing other agronomic characters significantly. By using Agrobacterium-mediated method, Ubi::AtMGT4was transformed into rice Zhonghua-11and the AtMGT4-ox transgenic plants were obtained, and subsequently, the characters of transgenetic plants were analyzed preliminarily.As the plant over-expression vector contain a marker gene AsRed, the transgenic calli will present a red colour at the screen stage. This label can easily discern transgenic calli from the non-transgenic calli at the very early stage, and is convinent to count the efficiency of transformation.By using Agrobacterium tumefaciens infecting the rice calli that were induced from mature embryos of ZhongHua-11seeds, we got the AtMGT4-ox transgenic plants. To improve the transformation ratio, we compared the efficiencies of different Agrobacterium tumefaciens strain (EHA105or Ag10), or different infection liquid medium (AAM or LB). The results indicated that Agrobacterium tumefaciens EHA105and LB infection liquid medium has a higer transformation rate.Transgenic rice were analyzed by PCR and SqRT-PCR, the results showed that AtMGT4had been integrated into rice and highly expressd in rice. There was no obvious difference in phenotype during the vegetative growth stage of plants.We campared the seed weight and size of AtMGT4-ox rice To generation transgenic plants with those of wildtype. The results showed that AtMGT4overexperss in rice can change the size of seeds, increase the content of Chlorophyll especially Chlorophyll A and the net photosynthesis rate while not affect plant's normal growth. |
