| The chemical constituents and biological actives of the fruiting bodies of D. ruhrovolvata and L. piperatus were investigated. Twelve known compounds were obtained using varied chromatography methods (CC and TLC), and the structures of the compounds were elucidated on the basis of extensive spectroscopic analysis (1D-NMR, MS, etc.). These natural products involve sterols, sesquiterpenoids, fatty acids, sphingolipids, and alkaloids.1. Seven compounds were isolated from the fruiting bodies of D. ruhrovolvata were elucidated as ergosterol (1),5a,8a-epidioxy-ergosta-6,22-dien-3β-ol (2),(22E,24R)-ergosta-7,22-dien-3β,5a,6β-trilol (3), octadecanoic acid (9), cerebroside B (10), cerebroside D (11) and uracil (12).2. Eight compounds were isolated from the fruiting bodies of L. piperatus were elucidated as ergosterol (1),(22E,24R)-ergosta-7,22-dien-3β,5a,6β-trilol (3),(22E,24R)-ergosta-7,22-diene-3β,5a,6β,9a-tetrol(4),3β,5a,9a-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (5), Isolactarorufin (6),7a,8β-dihydroxy-5,13-marasmanolide (7), and7a,8a-dihy-droxy-5,13-marasmanolide (8).3. The antioxidant activities of the ethanolic extracts obtained from the fruiting bodies of D. ruhrovolvata (Dre) and L. piperatus (Lpe) were investigated according to reducing power, metal chelating, DPPH·, O2-and·OH scavenging activity and inhibition of lipid peroxidation, and compared to standard antioxidant compounds such as a-tocopherol, trolox, BHA, and BHT. The antioxidant activities of Dre and Lpe were lower than that of antioxidant compounds, significantly. But the antioxidant activities of Dre and Lpe increased with concentration.4. Total phenolic and flavonoid compounds in the ethanolic extracts obtained from the fruiting bodies of D. ruhrovolvata and L. piperatus were measured. Total phenolics and flavonoids content of D. ruhrovolvata (2.74±0.38mg/g and1.305±0.03mg/g, respectively) lower than L. piperatus (5.20±0.46mg/g and1.430±0.05mg/g, respectively). |
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