Study On The Tobacco Ralstonia Solanacearum Strains Differentiation And Antagonist Bacteria In Hunan Province

As a devastating disease of tobacco, there haven't yet been any ideal chemicals that could control Ralstonia solanacearum so far, and using chemicals would not only pollute the environment, but also affect the quality of tobacco as well. Therefore, it's of great significance to find an effective biological control method.In order to diagnose quickly whether there is Ralstonia solanacearum in tobacco field soils, based on the genome-wide comparison results of five published Ralstonia solanacearum genome, this paper designs nine pairs of Ralstonia solanacearum-specific primers, and acquires three pairs of primers from them by selecting and verifying,which can cause specific amplification in Ralstonia solanacearum. These selected primers can be used to detect the specific of Ralstonia solanacearum in tobacco soil. As a preliminary conclusion, the lowest detection rate of Ralstonia solanacearum in soil by this method is2×106/g, which provides a rapid and accurate diagnosis way of detecting Ralstonia solanacearum in soil.By separating and purifying the tobacco with pseudomonas solanacearum which collected from Chenzhou, Liuyang, Shaoyang and other places in Hunan, this study obtains thirty Ralstonia solanacearum whose physiological races are all identified preliminarily as Race1. Thus provides the basis of breeding for disease resistance in tobacco.This study got two bacteria from the soil samples of Chenzhou tobacco field by separation and purification, which have strong antagonism against Ralstonia solanacearum.After studying and identifying, these bacteria both belong to Burkholderia,and as the molecular identification results shows, No.24is Burkholderia cepacia and No.88is Burkholderia vietnamiensis. Besides, the study also found that they have strong antagonism against Tobacco Black Shank as well. So in a conclusion, as the strains have antagonism against both Ralstonia solanacearum and Phytophythora parasitica, it will result in great economic benefits if used for biological control.