| The mastitis is a very harmful disease on milk production,which is highlyincidence and has a low cure rate. In order to study the mechanism to prevention andtreatment of mastitis, we observed interaction of the extracts of Lonicera japonica andStaphylococcus aureus on mammary epithelial cells to provide information. So we tookmethods of molecular biology to research the effects on goat mammary epithelial cellsof Lonicera japonica and Staphylococcus aureus.This experiment mainly includes thefollowing three aspects:(1) different concentrations of traditional Lonicera japonicaeffect the milk protein on goats mammary epithelial cells in vitro (2) goat mammaryepithelial cells have toxicity action to different amount of staphylococcus aureus liysisbuffer in vitro.(3) Lonicera japonica suppression inflammatory reaction which initiateby different amount of staphylococcus aureus liysis buffer on goat mammary epithelialcells in vitro. We got the following results:1Lonicera japonica extract at the concentration of1000μg/ml significantlypromoted the expression of beta casein (P <0.01).2Different amount of staphylococcus aureus liysis buffer influence expression ofgoat mammary epithelial cells,the expression of beta-casein was significant highcompared with control group when trained after48and72hours,the same change withthe three symbol enzymes of goat mammary epithelial cells damaged (P <0.01).3Different amount of staphylococcus aureus liysis buffer together with Lonicerajaponica extract at the concentration of1000μg/ml influence three symbol enzymes ofgoat mammary epithelial cells damaged, which was obviously lower than no Lonicerajaponica joined. It said that the Lonicera japonica has the effect to cure the damage ongoat mammary epithelial cells.The experiment results show that the staphylococcus aureus can causeinflammatory of goat mammary epithelial cells, Lonicera japonica extract caneffectively restrain the inflammatory caused by staphylococcus aureus. |
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