| Arsenic compounds exist widely with inorganic and organic arsenic form in nature. Theactual toxicity levels for different arsenic compounds vary greatly. Inorganic arsenic isconsidered the most acutely toxic form. The inorganic compounds of arsenic have beenclassified as carcinogenic. Arsenic contamination of surroundings caused serious harm to humanhealth. Now Arsenic contamination of bee products was also reported. Bee products were rich innutrition ingredients, trace elements and enzymes that human body needs, which were named as"Natural nutrients," and were diffusely development and utilized both at home and abroad. Whiletheir edible safety, especially the arsenic contamination caused great attention. Hydridegeneration and atomic fluorescence spectrometry(HG-AFS) together with high performanceliquid chromatography (HPLC) would become one of the main means of detection gradually inarsenic speciation analysis field at present, with its high sensitivity, low cost and simpleoperation. So the methods for determination of arsenic speciation in bee pollen and propolis byhigh performance liquid chromatography-hydride generation-atomic fluorescence spectrometrywere established in this study. The results were as follows:1. A method about the determination of arsenic speciation in bee pollen was established.The arsenic compounds, including arsenite(AsⅢ), monomethylarsonic acid (MMA),dimethylarsinic acid (DMA) and arsenate(AsV), in bee pollen were analyzed with highperformance liquid chromatography-hydride generation-atomic fluorescence spectrometry(HPLC-HG-AFS) as detection means and0.20mol/L sulfuric acid as extraction. The detectionlimits for AsⅢ, DMA, MMA and AsⅤ were1.0,2.1,1.2,3.1μg/kg, respectively, and therecoveries of standard addition were in the range of79%～100%with that the relative standarddeviations (RSD) were4.5%,2.3%,11.0%,7.7%, respectively.2. A method to detect the four arsenic compounds above mentioned in propolis wasestablished, with high performance liquid chromatography-hydride generation-atomicfluorescence spectrometry (HPLC-HG-AFS) as detection means, and with1%(V/V)phosphoricacid-methnol (1:1) as extraction.1%(V/V)phosphoric acid-methnol (1:1) and0.20mol/L sulfuricacid-methnol (1:1) were compared as extraction. It was found that the former had better stability,higher accuracy. The detection limits for As Ⅲ, DMA, MMA and AsⅤ were0.6,1.7,1.2,1.6 μg/kg, respectively, and the recoveries of standard addition were in the range of66%～110%with the relative standard deviations (RSD) all less than7%.The arsenic speciation analysis in bee products would provide science and technologysupport and the standard of appraisal for establishing safe and reliable bee products safetysystem and nutrition evaluation system. |
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