The Study On The Uptake And Accumulation Of Dehp In Benincasa Hispida

Food safety problems have been attended by more and more people with the improvement of life quality. Di-(2-ethylhexyl) phthalate (DEHP), which is the most commonly used among the phthalate esters, has been widely used as an additive in the manufacture of plastics and varnishes. DEHP can easily releases to environment as well as pollutes air, water and soil since it is not combined to plastics with covalent bond, The general population is ubiquitously exposed to this chemical, which is poses a potential risk to human beings through food chain.The uptake and accumulation of DEHP in Benincasa hispida were investigated in this paper. The distrubution of DEHP in the whole plant was clarified by determining the content of DEHP in the different section of the root, stem, leaves and fruit of Benincasa hispida. The The effects of the environment DEHP concentration, and the environment temperature on the DEHP accumulation as the increase of uptake time. It is useful for understanding the uptake pathway and transportation of DEHP in plants. The results are concluded as below:(1) The results of determination of the content of DEHP in the different section of the root, stem, leaves and fruit of Benincasa hispida showed that the DEHP content of the root is only1/11times of the adjacent stem. The content of DEHP in leaves from root to top decreased orderly. At the same part, the DEHP content in petiole, leaf and stem showed petiole> leaf> stem. The more tender leaves and stems the higher ratio of DEHP content for leave/stem, which suggested that the DEHP in leaves may tranfer to stem. The fruit pulp DEHP contents was1/3-1/5of those in peel, The part of fruit near pedicel accumulated higher DEHP content than the other part of the fruit. But the two ends of the fruit showed lower content of DEHP.(2) The effects of the environment DEHP concentration and the environment temperature on the DEHP accumulation and the maturity of fruit as the increase of uptake time on DEHP accumulation in the pulp, peel and leaves of Benincasa hispida were studied. The Results showed that temperature was the key factor which influenced on DEHP accumulation. The higher DEHP concentration in leaves and fruit peel the higher environment temperature and DEHP concentration. The concentration of DEHP accumulation in the pulp correlated positively to DEHP concentration in the peel. Transfer of DEHP from peel to the pulp of young fruit was relatively slower than mature fruit. (3) The ability of accumulation DEHP is different in the various parts of organs of Benincasa hispida. The content of DEHP in the nucleoprotein, chloroplast and mitochndria is higher while it is lower in the soluble substance and crude fiber, which suggested lipid constituents in nucleoprotein, chloroplast and mitochndria were key to DEHP accumulation. The leaves and peel have relatively high content of DEHP in the cell wall, which possibly caused of their epidermal wax. The high DEHP content of nucleoprotein and soluble part under centrifuge at100000g implicits lipoprotein and glycolipids is the carrier of DEHP transportation in Benincasa hispida The content of DEHP in root of Benincasa hispida is the lowest, which demonstrated the DEHP uptake pathway of Benincasa hispida is uptake from air of aerial parts.