SOD1 Model Compounds Regulate Antioxidant Activity In Cells Under Drought Stress Of Rice

Reactive oxygen species (ROS) are the oxidation of things with high oxidizability and reactive properties in general. ROS at a low level are conducive to cell signal transduction. Under the stress, plant organisms would produce a mass of ROS. ROS at a high level may disrupt the normal metabolism of organisms and even cause serious plant death. Antioxidant enzymes in plants are able to remove part of the reactive oxygen species in time. The appropriate exogenous antioxidants in biological tissue could reduce the level of reactive oxygen and regulating the antioxidant enzyme, in this way, oxidate damage could be reduced in plant.In this study, rice seeds were soaked in water containing three types of Cu/Zn superoxide dismutase (SOD1) model compounds [Cu(IDB)Cl2]·MeOH(1), [Cu(IDB)H2O](NO3)2(2), [Cu(IDB) H2O](ClO4)2-H2O(3). The activity of SOD in mimics was measured by NBT reduction method. Under drought stress, we detected O2-, H2O2 and HO·in rice, by UV-visible spectroscopy, fluorescence spectroscopy and fluorescence staining in situ. The activity of SOD,CAT,APX,GR in the leaf of rice were analysised by UV-visible spectroscopy, Native-PAGE, enzyme-linked immunosorbent assay.The main results in this paper including:1. The activity of SOD in mimics was measured by NBT reduction method, we found that the three mimics had high activity, their activity were as follow:3> 2> 1 by IC50.2. Reactive oxygen species under drought stress in rice were studied by UV-visible spectroscopy, fluorescence spectroscopy and fluorescence staining in situ. The results indicated that the content of ROS in rice decreased with the control, we found that reactive oxygen species in rice plants decreased gradually with increasing concentration of model compounds at a certain concentration range, and the rice soaked by model compound 3 contained the minimum oxygen content at the same concentration of the three model compounds.3. we detected the activity of SOD, CAT,APX,GR in the leaf of rice by UV-visible spectroscopy, Native-PAGE, enzyme-linked immunosorbent assay. We found that the activity of antioxidant enzyme in rice increased with the control, within a certain concentration range, the activity of antioxidant enzyme in rice increased gradually with the concentration of model compounds, and at the same concentration, the rice soaked by model compound 3 possess the highest activity.