| Members in the YABBY gene family play critical roles in determining organ polarity. In this paper, we had isolated a cDNA clone from Bambusa oldhami that named BoYABBY1 (BoYAB1). The results were as follows:(1) 766bp cDNA sequence of BoYAB1 was obtained, containing an open reading frame of 504bp coding 167 amino acids. BoYAB1 carried a zinc-finger motif in the N terminus and a YABBY domain in the C terminus, both of which were specific in the YABBY gene family.(2)Compared with the YABBY proteins in other plant species, BoYAB1 showed sequence identities to FIL(24.8%),GRAM(57.5%),OsYAB2(67.8%),TaYAB1(39.9%),ZYB9(37.2%),ZYB10 (37.5%) ,bphyem102c(47.4%),bphyem104c(45.2%),bphyem110b(26.6%),bphylf026i(32.1%),bphylf046c(46.4) and bphyst007p(45.8 %), respectively.(3)A phylogenetic tree was constructed based on amino acid sequence alignment. It showed that BoYAB1 wass more closely related to OsYAB1, OsYAB2, YAB2, YAB5 and PROL.(4) Transgenic plants were generated with the ectopic expression of BoYAB1 under CaMV 35S promoter in Arabidopsis thaliana. Compared with wild-type plants, the transgenic plants with mild phenotypes flowering time delayed about ten days, rosette leaves curled towards abaxial side, and the height was shorter. The transgenic plants with severe phenotypes could not bolting, some rosette leaves curled towards abaxial side and others curled towards adaxial side in contrast with wild-type plants.(5) To determine the expression levels of BoYAB1 in flower buds and vegetative shoots, real-time quantitative PCR was performed. The expression levels of BoYAB1 in flower buds was twenty-five times more than that in vegetative shoots. It showed that BoYAB1 might be involved in flowering of Bambusa oldhami.(6)In-situ hybridization experiment indicated that BoYAB1 transcripts accumulated in the stamen and carpel primordial. The results suggested that BoYAB1 may play a major role in maintenance of stamen and carpel. |
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