| Actinidia arguta subordinates to the genus of Actinidiaceae, Actinidia. Actinidia is a sort oflarge deciduous vine with rich content of nutritive value and medicinal value. The thesislaunched tissue culture technique of Actinidia arguta, so as to find the effective way ofimproving the quantity and quality of Actinidia arguta. And providing theorical and technicalsupport for production and germplasm conservation.Adopting tissue culture technology and uniform design, this thesis studied two paths toobtain regeneration shoots which directly come up from stem and indirectly from callus, furtherexplored the optimum regeneration medium and used stems cut from regenerated shoots for thepurpose of high efficient micro propagation. The conclusion was made as follows:1.The stem with axillary bud were used as explants and optimum medium compositions forstarting were screened through U10(103) uniform design, the results showed that the greatesteffect was6-BA, secondly was NAA, the basal medium type only have a negligible effect, Theoptimum medium was MS+NAA0.20mg·L-1+6-BA1.08mg·L-1for initiation induction with astarting rate of91%.2.Used MS+6-BA0.5mg·L-1+2,4-D1.0mg·L-1for induction medium and MS+6-BA2.0mg·L-1+NAA0.3mg·L-1for differential medium, studied different explants expect on theinduction and differentiation of callus, found that stem was the best explant of Actinidia argutacallus induction.3.Optimum medium compositions for Callus inducing were screened through U10(104)uniform design, the results showed that the greatest effect was IAA, secondly was6-BA, thesmallest effect was2,4-D.The optimum medium was MS+6-BA1mg·L-1+IAA2mg·L-1+2,4-D0.1mg·L-1for Callus induction with a inductivity close to one hundred percent.4.Optimum medium compositions for Callus differentiating were screened through theU10(103) uniform design, the results showed that the effect of6-BA is much larger than NAA.The optimum medium was MS+6-BA1.96mg·L-1+NAA0.3mg·L-1for Callus differentiation with adifferentiation rate of95%.5.Optimum medium compositions for axillary bud growing and rooting of stems werescreened through the U10(104) uniform design, the results showed that the greatest effect was IAA,secondly was IBA, the smallest effect was GA3.The optimum medium was MS+IAA1.96mg·L-1+IBA0.3mg·L-1+GA31.14mg·L-1with a regeneration rate of96%.6.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a5-fold proliferation rate was achieved within28days. High efficient micro propagationsystem of Actinidia arguta has been successfully established. 7.Selected healthy vitro seedlings, opening the culture bottle sealing film for3days duringseedling training. Substrate with rotting pine needles,peat soil and fine sand by2to1to1was theoptimum. The survival rate of transplants reached82.5%.Experimental indexes have reached the requirement of rapid propagation seedling, thetechnical measures could meet production needs. |
PDF Full Text Request