Construction Of Normalized Endosperm CDNA Library Of Maize And The Primarily Analysia On The Microarry Data

Corn is an important food crops, the utilization of heterosis in maize yield on their achievement. Although the mechanism of heterosis in a lot of genetic research, but for heterosis understanding of the molecular biological mechanism is still limited, further study of hybrid maize on Mechanism of great theoretical and practical significance.This study chooses the endosperm of maize, carried out a series of research, the main results obtained as follows(1) In this work, Saturation hybridization using genome can effectively reduce the high abundance gene in the proportion of homogenization library to achieve good homogenization effect.(2) 10368 articles longer than 500bp of PCR product. The PCR products were purified, produced chips, the data estimate the variation between chips and repeatability, the results show that the chip quality and meet the requirements, can be used for subsequent hybridization. gene expression of information research corn endosperm gene expression provides a basic platform.(3) Inbred corn self consolidated 11,16,20 days after pollination, endosperm (control group) and the Comprehensive 11,16,20 days after pollination the endosperm (as maize pollen pollination 87-1 experimental group) for gene chip hybridization materials. Test results show that 11 days after pollination endosperm (experimental group) increased the expression of clone 303, down 36 expression cloning; 16 days after pollination endosperm increased expression clone was 49, down 29 expression cloning; hybrid the first 20 days of pollination endosperm expression clone for the 503 up,274 down-regulated expression cloning. Pollination endosperm increased number of clones expressed differentially expressed clones accounted for 71.6% of the total.28.4% reduction in expression.(4) in these differentially expressed genes, ZMABI4, MADS-BOX-29 and 22 kDa zein and grain weight, which has close relationship between regulation relationship, constitute of corn kernels gene regulation network control weight. ZMABI4, MADS BOX-29 and 22 kDa zein in hybrid and the differences between the parents may express heterosis formation.