Expression Of KatA And KatB Gene And Adversity-tolerance Identification Of Transgenic Plants In Tobacco

Catalase (EC1.11.1.6) is the one of the most important antioxidant enzymes in vivo, which widespread in animals, plants and microorganisms. The physiological function of catalase is to catalyze hydrogen peroxide decomposition, Which plays an important role in stress tolerance. NC89 tobacco plants were transformed via Agrobacterium tumefaciens, PCR and RT-PCR analysis indicated that the foreign gene KatA,SP-KatA,KatB of P.Aeruginosa had been integrated into the genome. Analyse the expression of KatA and SP-KatA gene by semiquantitative RT-PCR. And then adversity-tolerance analysis was done by water stress, salt stress and H2O2 stress. The comparison was done between KatA tobacco and SP-KatA tobacco.The results prove that on the 9th day of water stress, the 9th day of 200 mmol/L Nacl stress and the 23th day of 0.3 mm/1 H2O2 stress, the tissue relative water content, MDA activity, relative conductivity and relative quantum yield of PSII of KatA tobacco are superior than control tobacco evidently. Transgenic plants have more tolerance to adversity. Which testify that KatA gene has a better application prospect on the adversity-tolerance genetic engineering.On the 13th day of water stress, the 19th day of 200 mmol/L Nacl stress and the 27th day of 0.3 mm/1 H2O2 stress, the tissue relative water content, MDA activity, relative conductivity and relative quantum yield of PSII of SP-KatA tobacco are superior than control tobacco and KatA tobacco evidently. Transgenic plants have more tolerance to adversity. which testify that SP-KatA gene has a better application prospect on the adversity-tolerance genetic engineering.On the 13th day of water stress, the 20th day of 200 mmol/L Nacl stress and the 33th day of 0.3 mm/1 H2O2 stress, the tissue relative water content, MDA activity, relative conductivity and relative quantum yield of PSII of KatB tobacco are superior than control tobacco evidently. Transgenic plants have more tolerance to adversity. Which testify that KatB gene has a better application prospect on the adversity-tolerance genetic engineering.We obtain 8 KatA tomato seedlings and 2 SP-KatA tomato seedlings. Under the same conditions, there was no significant difference between transgenic tomato seedlings and wild tomato seedlings.