The Molecular Biotype Identification And Researching Relations Between Their P450 Gene And Host Conversion Of Bemisia Tabaci

Bemisia tabaci is one of the most seriously invasive pests worldwide, and has already been a major insect pest in Hubei Province. At present, the technique of mtCOI PCR-RFLP is widely used in identification of different whitefly biotypes worldwide.In this paper, using mtCOI based PCR-RFLP and gene sequencing method, we identified the biotypes of whitefly field populations from main cotton planting areas in Hubei Province, and the phylogenetic analysis of them was also conducted. In this paper, we compared the utility efficiency of ever-reported five mtCOI based PCR-RFLP techniques in differentiating the invasive and local biotypes (B, Q, ZHJ1, ZHJ2 and ZHJ3) of China. In this paper, for obtaining host adaptability differences, we use the methods of host conversion application, the technique of half quantitative polymerase chain reaction (PCR) and fluorescence quantitative polymerase chain reaction (PCR) to analysis the host adaptability of Bemisia tabaci to change cytochrome P450 gene expression quantity differences. Main results are as follows:1.The Q biotype is dominant in the field populations, while the B biotype is dominant in greenhouse populations. In addition, ZHJ1 biotype was also identified in Wuxue population. Phylogenetic analysis showed that populations from main cotton planting areas in Hubei Province were always homologous with populations from most of other provinces in China.2. Utility of mtCOI PCR-RFLP in differentiating invasive and local whitefly biotypes of China. The enzyme Alul fails to differentiate between B and ZHJ2 biotypes; enzyme TaqI could not effectively differentiate between ZHJ3 and Q biotypes; enzyme VspI is not effective in differentiating between neither B and ZHJ2 biotypes, nor Q and ZHJ1 biotypes; and the enzymes MseI and Tru9Iare completely unable to identify any of the five biotypes of China.3. Cloning part genes of family P450, I do half quantitative polymerase chain reaction (PCR)and select after adaptability to change the amount of CYP4 differentially expressed genes from cloning P450 genes in Besimia tabaci.