| The Antarctic krill oil has the extremely high nutrition and the medicine value. Related research found that Antarctic krill oil could regulate the serum lipids, hyperglycemia and Nonalcoholic fatty liver. The functions of Antarctic krill oil health are comprehensive, long-term and stable, with little side effects. Therefore, applications of the Antarctic krill oil on food, medicine, industry and agricultur are widespread. However, the research of Antarctic krill oil is still only under way. The development of Antarctic krill oil deep processing technology, especially the development of high-purity Antarctic krill oil preparation method has important economic and social value in China.This article has studied the extraction of Antarctic krill oil with alcohol and Immersion mention, distillation, drying, and then obtained the Antarctic krill oil of high purity. Based on the above study, both the effects of the Antarctic krill oil on the serum lipids and antioxidation ability in hyperlipidemia rats and the improvement of mouse memory with Antarctic krill oil were evaluated. This research was divided into three parts.Part 1: The Antarctic krill oil extraction and fatty acid composition analysisUsing alcohol extracted Antarctic krill oil from krill, took frozen krill dried in 75℃hot air and then machine shattered, put krill powder immersed in 65℃alcohol for 3 hours,finally, Antarctic krill oil can be obtained by distillation from Supernatant fluid.Part 2: Effects of the Antarctic krill oil on the serum lipids and antioxidation ability in hyperlipidemia ratsAfter feeding with high fat diet,the hyperlipemia model rats was established, then the rats were given by ig at different doses of krill oil, 50, 100 and 500 mg·kg-1 respectively for 30 days. The contents of total cholestero (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), NO,superoxide dismutase (SOD) , Glutathione peroxidase ( GSH-PX) and the content of malondialdehyde (MDA) were determined.Part 3: Antarctic krill oil improves learnings and memory ability in ratsWistar rats drink and eat freely in three days in order to adapt to the environment.All the rats are divided into six groups by weight: blank group, deep fish oil group, Antarctic krill oil low, medium, and the high-dosage groups, positive control group. Groups began to fill corresponding drugs for 60 days and weighing weekly. By Morris-water maze and Y-maze methods, the effects of Antarctic Krill oil and fish oil on learning and memory ability in rats were observed.Results: The Antarctic krill oil could decrease the content of TC ,TG and LDL-C in serum significantly, decrease the atherogenic index (AI)markedly, increase serum NO and the activities of serum SOD and GSH-PX significantly, Meanwhile, decrease MDA obviously. The Antarctic krill oil can regulate the serum lipids and increase antioxidation ability in hyperlipidemia rats, and it's much better than fish oil. The Antarctic krill oil contains abundant EPA and DHA, phospholipids, flavonoids, vitamin A, vitamin E and astaxanthin. EPA and DHA phospholipids in omega - 3 form existence, different from cyclooctene fish oil and cod liver oil three triglyceride in omega– 3 form; Antarctic Krill oil could significantly reduce the time of escape latency, increased the number of the crossing platform frequency and the proportion of the time of swimming in the target area in rats in Morris-water maze; Y-maze's results indicated that Antarctic Krill oil could significantly reduce the rats standard required number and improved the correct response rate after 48 h. The Antarctic krill oil can improve learning and memory ability in rats, and it's much better than fish oil.But the exact molecular mechanism of the Antarctic krill oil can regulate the serum lipids and increase antioxidation ability and improve learning and memory ability is still to be confirmed. |
