| Compound amoxicillin powder is the laboratory developed by streptococcus disease prevention and controls the compound pig, is by forsythia extract and amoxicillin and of accessories. For rage of plants suspensa (Forsythia suspensa(Thunb.)Vahl) dry fruit forChina pharmacopoeia monographs, the commonly used Chinese traditional medicine, have anti-inflammatory, antibacterial, antiviral effect. Amoxicillin, alias hydroxyl ampicillin penicillin, belongs to theβ-lactamase antibiotics, for the compound drug residues, the main research targets, the European Union and China's ministry of agriculture in the edible organizationregulations are the highest residue limits for 0.05 mg/kg.In order to keep this compound medicine used standardly in hogpen, keep human body health, develop animal husbandry and promote the product import, it is necessary to establish residue monitoring system and withdrawl time.A ultra performance liquid chromatography coupled with electrospray ionization tandemmass spectrometry for determination of amoxillin residues in swine edible tissues has beendeveloped. Tissues are extracted with acetonitrile and water (15/2, v/v), purification by HLBSPE cartridge, eluted by acetonitrile. The linear ranges of amoxicillin sodium is between0.01~1μg/mL,and the linear correlation coefficient is 0.9993~0.9995.The recoveries of amoxicillin at the spiked concentration levels of 0.025 mg/kg,0.05 mg/kg,0.1 mg/kg ranged from 63.4±4.11%~74.1±4.18%,with RSD of 5.2%~6.4%. Within day CV is 5.9%~7.6%,between day CV is 5.3%~12.4%.The limit of detection is 0.01μg/g, the limit of quantitation is 0.03μg/g.Select the 15 piglets weighing 20 kg, a dose of oral administration of amoxicillin sodium 2 mg/kg.bw, forsythia 8mg/kg.bw, continuous administration of 7 days, after withdrawal for 0h,The results indicated: the concentrations of amoxicillin residue in kidney is highest, it is 0.0409±0.0001 mg/kg,the concentrations of amoxicillin in muscle and fat,liver,lung,heart is lower than MRL in turns; after withdrawal for 12 h, amoxicillin in heart and liver can't be detected,the concentrations in lung,kidney,muscle and fat is 0.0110±0.0001 mg/kg,0.0277±0.0001 mg/kg,0.0280±0.0001 mg/kg,respectively, and the residual amount is lower than MRL; after withdrawal for 24 h, amoxicillin in all the tissues can't be detected. Accord ing to the usual methods of calculation for the withdrawal time in our country, the withdrawal time for this compound medicine was advised as 0 h in pig. Select the 15 pig lets weighing 20 kg, a dose of oral administration of amoxicillin sodium 2 mg/kg.bw, continuous administration of 7 d, after withdrawal for 0 h, the results showed: the concentrations of amoxicillin residue in kidney is highest, it is 0.0457±0.0001 mg/kg, the concentrations of amoxicillin in muscle and fat,liver,lung,heart is lower than MRL in turns after withdrawal for 0 h; after withdrawal for 12 h, amoxicillin in heart can't be detected, the concentrations in liver ,lung, kidney, muscleand fat is 0.0110±0.0001 mg/kg,0.0113±0.0001 mg/kg,0.0303±0.0001 mg/kg,0.0257±0.0001 mg/kg, respectively, and the residual amount is lower than MRL; after withdrawal for 24 h,amoxicillin in all the tissues can't be detected. According to the usual methods of calculation for the withdrawal time in our country, the withdrawal time for this compound medicinewas advised as 0 h in pig. From the results, Forsythia in the combination drug amoxicillin extract residues in pig tissues and metabolism of less affected, in addition to 12 h unilateral compound amoxicillin compared with the residues in the liver, significant differences between the other time points Residues of the organizations, compared with unilateral no significant difference. |
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