| With local white Cyathula officinalis Kuan seed as material. By a field experiment in lianglu of Tianquan town. Studied the impact of 1-3 years old Cyathula officinalis Kuan growth dynamics and plant density (number of acres and number of litter) on Cyathula officinalis Kuan dry matter accumulation and distribution, production and polysaccharide, the quality indicators like content of cyasterone and so on.1. In the same year, Cyathula officinalis Kuan prouting and growth in In April or May, which has fastest growth in August or September,the leaves start turning yellow in October off and aboveground down seedlings after the November. With years of growth, plant height, leaf area, root number, root length and root diameter gradually increased, dry weight increased. Stems growth rate of annual Cyathula faster than the leaves, but from the second year the growth rate of leaves fater than stem. The first and the second year Cyathula root mainly is root elongation of root length and increase in the number, the third year Cyathula root diameter growth faster. During the same period the root, stem and leaf growth is higher than the previous year.2. Within a year, a percentage of leaf dry weight accounting for the whole plant increase first and then decreased, the highest in July per year.The percentage of the root dry weight constant increase in the first year, which decreased first and then increased after two years.3. Annual dry weight per plant and the organ decreased with the increase of planting density, but the population biomass on the contrary. Distribution of photosynthetic products to the main stem in the most productive period August, followed by root. The rate of leaf distribution is relatively low; The impact of planting density on the 2,3-year old Cyathula dry matter accumulation and distribution is Smaller than one-year old. Like one-year old Cyathula, group dry weight and plant density are positively correlated. The dry matter mainly allocated to roots in August, followed by stems, leaves the distribution rate is lowest still. The N, P, K content of Cyathula organs, one-year old> two-year old> three-year old, leaves> stems> roots.4. Yield per acre of Cyathula increased with the increase of the number of nests per acre, decreased after increasing with the increase in the number plant of nest.The number of nests per acre and in the number plants of nest interacte significant, in which A2B2 was the best. That is, when 10,000 litter/acre and leaving 7 plants per nest,the highest yield is 814.13Kg/acre, A1B1 is lowest yield for 431.45kg/acre. By single factor experiments and orthogonal test method, the study draws the best ultrasonic extraction process of Cyathula polysaccharide is:Liquid ratio of 1:60, extraction 30min, extracted one time, extraction temperature is 50℃. The stability, re-producibility, recovery test results show that the extraction process is feasible. Determined by the results of this process show that:Cyathula polysaccharide of two years old Cyathula> one year> threeyears. The impact of planting density on the polysaccharide content of three-years old Cyathula is significant. The content of polysaccharide decreased with the increase in the number of nests. The highest content of polysaccharide are A1B3 and A2B2, A3B2 is the lowest.5. Established by Cyathula fingerprint HPLC. Generated the control map by HPLC fingerprint, extracted 8 peaks from them.By comparing the standard cyasterone, determining the 6th is cyasterone peak. Calculated the contents of cyasterone of every year and every handle Cyathula based on peak area. The results showed that:the content of cyasterone of one-year old Cyathula is higher tan two or three. The content of cyasterone of three-years old Cyathula and planting density have not significant effect. |
