| Ginseng is a famous traditional medicine of China,which is widely used in the health-care cosmetics because of its special effectiveness.The main ingredients of ginseng are ginsenosides, and the dammarane type ginsenosides own the highest content,which can be hydrolyzed to panaxadiol and panaxatriol,and they are commonly used to evaluate the quality of ginseng products by content.Panax ginseng as a main function additive for the health-care cosmetics,its contents have a direct impact on the quality of cosmetics.The basic principles and applications of high-performance liquid chromatography(HPLC), the present developments and applications of ginsenoside by HPLC,and the qualitative and quantitative analysis of ginsenosides were reviewed in detail in the first part of this paper.The technological parameters of ginsenoside acidic hydrolysis to panaxadiol and panaxatriol were researched,and the optimum conditions were 70%of ethanol solution as solvent,7%of hydrochloric acid as the acidifier,the reaction temperature was 80℃,the hydrolysis time was 3 h.The conditions of derivatizing reaction for panaxadiol and benzoyl chloride were researched, and the optimum reaction conditions for the derivative were 3 times the amount of benzoyl chloride and 2 times the amount of pyridine,the derivatizing reaction temperature was 80℃,the time was 2 h.A pre-column derivatization method was established in the paper for the determination of panaxadiol and panaxatriol in cosmetics by RP-HPLC.The method was carried out on a TECHCOMP LC2130 system equipped with a UV detector at wavelength of 230nm,the column used in this research was a C18 column(4.6×150mm,5μm) and the column temperature was around 25 8888888,the mobile phase was CH3CN-H2O=40:60(V/V) and the flow rate was 1.0mL·min-1. The contents of panaxadiol and panaxatriol were calculated by external reference method of peak area.The experimental results were showed that the contents of panaxadiol and panaxatriol had a good linearity with the peak area in the range of 0.01-0.10mg·mL-1.The equation of linear regression of panaxadiol was y=3087735x+324849,the correlation coefficient was 0.9996,the RSD of peak area was 1.61%(n=6,c=0.06mg·mL-1),and the recovery was from 98.2%to 103.6%, the detection limit was 0.07μg·mL-1.The equation of linear regression of panaxatriol was y=45362537x+383509,the correlation coefficient was 0.9977,the RSD of peak area was 2.84% (n=6,c=0.06mg·mL-1),and the recovery was from 96.5%to 101.7%,the detection limit was 0.06μg·mL-1.After extraction,hydrolysis,derivatization and other operations,the contents of panaxadiol and panaxatriol were panaxadiol 52μg·mL-1 and panaxatriol 39μg·mL-1 in the ginseng extract,panaxadiol 22μg·mL-1 and panaxatriol 17μg·mL-1 in the ginseng cosmetics respectively. The peak retention time of panaxadiol derivatives was about in 11min,panaxatriol derivatives was about in 16min.This method was proved to be a perfect one,which was rapid,sensible and feasible in the evaluation of ginseng quality,and with a great significance in quality controlling of cosmetics. |
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