Research On Processing Technology Of Pearl Oyster Peptide-calcium And Plant Design

At present, most of the clam meat was abandoned directly, causing great waste of resources.Fresh mussel meat contains10.9%protein,is one of the best raw materials to prepare peptide calcium. Peptide-calcium was prepared by peptide chelating with calcium, the calcium ions were transported and absorbed in it's chelating state in hunman body and released when it is needed in the form of calcium ion, peptide calcium as calcium products not only can promote the body's absorption of calcium, but also can supplement the polypeptide.This researsh is based on the river mussel Yo bead after the clam meat as raw material, using enzymatic hydrolysis, ultrasonic modification, chelating technology to prepare the clam peptide calcium, and joined the conventional excipients and vitamin supplements by compound to preparation of calcium,and designed a plant which can product50tons of calium each year. The results are as follows:(1) The optimal process of peptide calcium was determined through single factor experiments and orthogonal experiments.Feed liquid ratio is1:30, chelating temperature is35℃, chelating time is45min, peptide calcium ratio is1:1.5, pH is9.5. Under these conditions, the calcium mass fraction in peptide-calcium is3.15%.(2) Through the study in ultrasonic modification process, we confirmed the conditions of modifying Ultrosonic power160W,temperature50℃,time10min, The concentration of peptide of1:40. Under these conditions, the calcium mass fraction in peptide-calcium is6.037%.(3) The chelating reaction of modified peptides and calcium ion was optimized.The conditions are as follow:peptide calcium ratio1:1, The concentration of peptide1:40, temperature40℃, pH9, time30min. Under these conditions, the calcium mass fraction in peptide-calcium is6.307%. The chelation of modified peptide calcium is better than that of without modifying.(4) Using infrared spectrum and ultraviolet spectrum scanning techniques to analyze the preliminary structure of peptide-calcium, the results showed that the ultraviolet absorption peak shift after chelation, the characteristic peaks of carboxylate appeared in infrared spectroscopy. These features indicate that peptides and calcium have chelated. Through the scanning maps of electron microscopy, we can see that polypeptide and calcium ions generated small particles aggregation group through ion, coordination bond and so on, and formed a structure which similar to salt. Thereby, we can move a step to prove the chelation between polypeptide and calcium ion.(5) After make a plane design on the calcium production workshop, we obtained the workshop layout, at the same time, we also got the production fee (raw materials, water, electricity, steam,artificial) which is4,147,000RMB after estimating the cost of material, water, electric, steam in the process of the production. Sales revenue of8,300,000RMB.