The Study Of Matrine Extracti On Methcd

In this paper, according to the single traditional chinese medicineSophora flavescens extract of Radix Sophorae Flavescentis characteristics, thewater extraction, ethanol extraction, extraction method were systematicallystudied by using L9(34) orthogonal test design by matrine in differentextraction solvents and extraction methods the optimum extractionconditions.Screening of matrine water extraction process conditions,3friedwater were:10times,8times,8times, with total time:5h, followed by3h,1h,1h, size of material for the10-20meshes. Matrine ethanol extraction processconditions, ethanol concentration of40%,3fried ethanol dosage were:8times,6times,6times, with total time:2h, followed by1h,0.5h,0.5h, size ofmaterial for the50-80meshes. Microwave extraction method of matrineextraction conditions,3fried solid-liquid ratio were:6times,4times,4times,with total time for3min:2min,0.5min,0.5min order, size of material for the10-20meshes, radiation frequency is600Hz.According to screen the best process conditions of Sophora flavescens,comparison of water extraction, ethanol extraction and microwave extractioneffect. With matrine, total matrine and dry extract percent as the content index,comprehensive evaluation of the results Y microwave extraction=0.7004Y ethanol extraction=0.1850Y water extraction=-0.88700So experimentalverification proved that the optimum process conditions are stable andreliable.By3kinds of extraction methods of in vitro for fingerprint ofmicrowave extraction, fingerprint and other extracts similarity size waterextraction ethanol extraction0There are twelve characteristic peaks inmicrowave extraction, eleven characteristic peaks in water extraction, ninecharacteristic peaks in ethanol extraction, With the microwave extractcharacteristic peaks corresponding to the total area of characteristic peaksfrom big to small order microwave extraction(41808727) water extraction(34165713)ethanol extraction(31694822).There are six characteristicpeaks in medicated serum of microwave extraction, five characteristic peaksin medicated serum of water extraction, five characteristic peaks in medicatedserum of ethanol extraction, With the microwave extract characteristic peakscorresponding to. the total area of characteristic peaks from big to smallorder microwave extraction5205839water extraction(4730797)>ethanol extraction(4445779). And through the bacteriostatic test in vitroconfirmed, microwave extract against Staphylococcus aureus and Escherichiacoli bacteriostatic effect is best, Staphylococcus aureus antibacterial ringdiameter microwave extraction (11.5mm)> water extraction (9.8mm)>ethanol extraction (8.4mm) Escherichia coli bacteriostatic ring diameter ethanol extraction (9.7mm)> ethanol extraction (8.5mm), Microwaveextraction efficiency was significantly higher than the other two methods.By three methods for extracting fingerprint determination, proves thatmicrowave from Sophora in the general components and single flavorcomponents extraction efficiency is better than the other two methods.