Oxidation Of Chlorophenol Catalyzed By Fe-based Phthalocyanine And Its Application

Chlorophenol compounds are not only a kind of impotant industrial organic compounds, but also pervasive environmental pollutants. They are widespread, toxic and difficult to degrade.2,4-dichlorophenol (DCP) are a kind of familiar chlorophenol compounds, which are extremely dangerous to the environment and human health. Therefore, the detection of DCP concentration is of crucial importance to environmental protection. Fiber optic biosensor is one of the latest developments of biological detection technology. Fiber optic biosensor based on enzyme catalysis has been widely applied in many fields, such as environment monitoring, food engineering, clinical medicine, etc. However, the application of the natural enzyme has been limited because of their poor stability, difficult extraction and purification, and very limited source. The researcher's attention has been attracted by metallophthalocyanine as biomimetic enzyme due to its stable chemical properties, excellent catalytic ability and effective cost. In this thesis, metallophthalocyanines had been prepared and were used as biomimetic enzyme to catalyze the oxidation of DCP The factors to influence the catalytic activities of metallophthalocyanine were investigated. A fiber optic DCP biosensor based on metallophthalocyanine catalysis and fluorescence quenching was designed and constructed, and it has successfully been used to detect the concentrations of DCP.In this thesis, the systematic studies are conducted as follows:First, Fe-based phthalocyanine (Fe(II)Pc and Fe(III)PcS4) were synthesized and used to catalyze the oxidation of DCP. The factors to influence the catalytic efficiency, such as the amount of phthalocyanine, the pH value of solution and temperature were investigated. Second, a novel fiber optic DCP biosensor was designed and constructed, and the performance of the novel biosensor was studied. The main research and results are as follows:1. Fe(II)Pc was synthesized with phthalic anhydride and urea. The reaction time was shortened by using dimethylbenzene in place of high boiling point solvent, and it was easy to get the products. Fe(III)PcS4was prepared by solide phase method. Both Fe(II)Pc and Fe(III)PcS4have high purity and good crystallinity.2. In phosphate buffer solution (PBS), Fe(II)Pc could effectively catalyze the oxidation of DCP. The corresponding oxidation products was pink dye. The optimal pH and Fe(II)Pc amount for the oxidation reaction of DCP are pH7.0and0.035mmol, respectively.The optimal temperature is25℃. Oxygen was used as the oxidant.3. Fe(III)PcS4could effectively catalyze the oxidation of DCP as well. The optimal pH and Fe(III)PcS4amount for the oxidation reaction of DCP are pH6.0and7.0×10-4mol/L, respectively. The optimal concentration of4-AAP is twice as much as the concentration of DCP. The optimal temperature is25℃. The influence of amount of oxidant (TBHP) to the catalytic efficiency was investigated.4. The performance of the fiber optic DCP biosensor based on Fe(II)Pc catalysis was investigated:A good linear relationship between A<p and DCP concentration is observed in the range of1.0×10-6-9.0×10-6mol/L and1.0×10-5-9.0×10-5mol/L. The sensor has the detection limit of7.0×10-7mol/L and the response time of300s. It has good stability and repeatability.