The Study Of Structure And Bioactivity Of Polysaccharide Extracted From Viscera Of Abalone

We extracted active polysaccharide from Abalone viscera which can provide theoretical and technical support to increase the added value of abalone viscera, and to promote the utilization and development of abalone. The polysaccharide of CAVP was isolated from Abalone viscera by water.The water-extracted polysaccharide CAVP was fractionated by frozen-thawed, deproteinated with enzyme/Sevag method and purified by gel filtration chromatography on a column of Sepharose CL-6B. Then fraction were obtained and named as AVP. The content of polysaccharide was 91.24%. The molecular weight of it was 3.5×104 Da ,which measured by HPLC. GC analysis showed that AVP was composed of Man,Gal and Glc.The molar ratio was2.7:0.7:6.5.The structure of AVP was elucidated by periodate oxidation, Smith degradation, methylation analysis and IR,GC,GC-MS. The partial acid hydrolysis of AVP showed that the main chain of AVP was composed of Glc .The end of sugar chain or branched-chain was composed of Gal and Glc. The methylation analysis show that AVP consisted of a backbone was composed of (1→4)-Glc The side chains was composed of (1→3,6)-linked–Man non-reducing terminal residues was composed of (1→)-Gal and (1→)-Glc.The immunobiological activity of CAVP and AVP were detected by Lymphocyte proliferation assay with MTT. The bioassay showed that CAVP and AVP could induce Lymphocyte proliferation from 100μg/ml to 400μg/ml, and the higher the concentration, the effect is more obvious. In the dose of 100 and 200μg/ml, CAVP and AVP can promote the ConA-induced proliferation of T Lymphocytes, what is more, they did affect the LPS-induced proliferation of B lymphocytes.