| Sulfonamides is one of the largest production and use of veterinary drugs, however, the utilization was low in animal,Less than 50%,Most of the original form of drug or metabolites excreted with animal dung to the nature,caused great harm On the ecological environment,especially in the soil environment.Sulfa drugs will be interacted with heavy metal pollutants,that may cause more serious consequences.For evaluation the potential risk in ecological environment of combined pollution between Sulfonamides drugs and heavy metals contamination,study the eco-toxicity on soil and plant of combined pollution between sulfamonomethoxine (SMM) and cadmium (Cd)。Using potting to study the impact of combined pollution between SMM and Cd to soil enzyme activity on soil - soybean system.The results showed that cadmium could outstanding inhibited the activity of phosphatase and invertase,early in the trial SMM also could outstanding inhibited the activity of urease, catalase, phosphatase, but in the late in the performance of weak activation,and SMM plays the role of Inhibition invertase activity continued to the end of the experiment.When SMM concentration was constant, the combined treatment on the inhibition rate of phosphatase activity with increasing concentration of cadmium in larger and later in the test cycle, the various enzyme activity also show such circumstances, this may be due to impact of enzyme activity by cadmium was large and persistent, the effects of SMM on the enzyme activity in the latter part of the experiment weaken because of theirself concentration and microbes resistant。Studies of Cd-SMM combined pollution on ecological toxicity of seedling showed that in single and combined pollution of stress of Cd or SMM, soybean seedlings of chlorophyll, MDA and soluble protein content, and superoxide dismutase, peroxide physical activity of the enzyme had great changes throughout the experimental period, and changes in the root greater than in the leaves. Plants by the Cd-SMM single or multiple pollution, will be significantly reduced chlorophyll content, a serious cause yellow leaves; addition to individual treatment, Soluble protein content on combined treatment of Cd and SMM was lower than the blank; MDA content in leaves in the test period increased gradually over time, and higher than in the blank treatment. Low concentration of compound pollution of stress will increase the peroxidase and superoxide dismutase activity, but high concentrations of combined pollution will reduce both the activity of enzymes.In the late of experience, addition to individual high concentration treatments, enzyme activity of other treatments were no significant difference in the blank.Results of Cd-SMM combined pollution on seedling growth showed that in Cd-SMM combined pollution stress, growth of plant was inhibited. Impact of combined pollution on plant height and stem weight were similar, when the concentration of Cd is constant, they increased with decreased concentration of SMM, and the height and stem weight on the combined pollution of Cd and SMM were letter than single treatment. Damage on soybean roots and leaves are larger. High concentrations of combined pollution of Cd and SMM will greatly reduce the plant's root weight.Single SMM treatments can significantly reduce the plant's leaf weight, presence of low concentrations of Cd in some extent stimulate the growth of leaves, mainly because low concentrations of Cd can promote the growth of chlorophyll, while high concentrations inhibit growth, so combined treatment of high concentrations of Cd and SMM significantly reduced the plant's leaves weigh. |