Fungus Screening Of The Allylic Hydroxylase And Researching Of The Preparation Of Biotransformation 1α-Hydroxy Vitamin D

The active Vitamin D is suitable for the rickets in the prevention osteoporosis sickness, the parathyroid function hyperfunction as well as the decrease, the nutrition and the absorbing barrier cause the chondrification sickness, the pseudo lack the calcium caused the rickets and the chondrification sickness and so on. It is the metabolite of the human body's liver and kidney, but the work of extracting these materials from the biological tissue is too huge, receiving rate is extremely low, obviously is unable to meet the clinical need.The chemical synthesis method need carry on many step groups and bases protections and escape the protection, responded using the illumination, the split-ring and the isomerization, so much the synthesis steps, so complex the separation purification, so low the synthesis receiving rate.This article uses biological conversion method preparation active Vitamin D, Has laid the foundation for the biological preparation active Vitamin. The biotransformation can carry on the response which certain chemistry method difficulty with carries on or cannot synthesize, the product spatial configuration is unitary, and the transformed condition is moderate.First we screened the fungus of allylic hydroxyl from the nature gathering sample,obtained four growth good shapes, strong ability of transforming strains.. Has conducted the strains appraisal research and carried on the research of using the fungus biotransformate the preparation of active Vitamin D.In the conversion process,we uses the thin layer chromatography to transmutation product's examination (TLC) and highly efficiency liquid chromatography (HPLC). After prepares HPLC, collect transmutation product, Through the nuclear magnetic resonance hydrogen spectrum and the mass spectrum examination, The results show that:The transmutation product of purification obtains's structure is consistent with Standard's structure.