Nutritional Components And Extraction , Separation Of Loligo Japonica Polysaccharides

Teuthoidea, is a Marine ten wrist cephalopods molluscs collectively. It belongs to Mollusca,Cephalopoda, Teuthoidea, Loliginidae, which is a special of sea anemone found in seaside in the yellow sea and bohai sea of China. With the development of glycol-biology, polysaccharides isolated from various sea animals have a great deal of attention because of the anti-tumor activity, anti-virus activity, antioxidant activity immunoregulating activity and relatively low toxicity. In this paper, extraction, isolation and structure analysis of polysaccharides from Loligo japonica were investigated that provide reference for utilization and exploration of Loligo japonica.This paper analyzes the protein and fat in the Teuthoidea mainly through the nutrition analysis method and assays 17 kinds of common constitutes a protein content of amino acids and main fatty acids. The results show that Teuthoidea is high protein, low adipose seafood; more than 50% of dry-out sample is protein, essential amino acids and non-essential amino acid content is balance, thus Loliginidae protein amino acid composition is accord with the FAO/WHO recommend mode. Fatty acid analysis shows there is rich PUFA in Loliginidae.According to the single factor experiment and orthogonal experiment, the optimal extraction parameters are established as follow: ratio of material to solvent is 1:3 (w/v); extraction temperature is 50℃; enzyme load is 7.5%; and initial pH is 9.In the purification study, compares the different methods of removing proteins,through comprehensive consideration of protein as the index, Chose trimethoxysilane n-buoh acetic acid-laws as Deproteinization method, Verify lye before enzymatic hydrolysis can effectively promote to release of polysaccharides.Chemical methods are used to analysis the constituents of Teuthoidea, it show that the glycuronic acid is13.53%. HP GPC analysis shows that the molecular weight of Actiniaria Purificata is 162039. IR spectrum indicate tha Teuthoidea polysaccharides configuration for Beta much crystalline, polysaccharide monosaccharide glucose is D-pyranoid sugars ring configurations. Through its composition analysis, found polysaccharide of Teuthoidea is mainly composed of D-Man and D-Fuc, there is also some D-(+)-Gal Hcl, GlcN, D-Glu, D-Gal, GluA...